Decline of Yezo spruce (picea jazoensis (Sieb. et Zucc.) Carr.) forest has been recently noticed in Hokkaido. In this experiment comparison of microorganisms isolated from the Yezo spruce roots from declining and healthy stands was obseved. Fine roots of Yezo spruce were collected from declining and healthy stands in the Tokyo University Forest in Hokkaido, Furano, Japan. Soil-free roots were homogenized in sterilized water and the number of total bacteria, nitrogen-fixing bacteria, actinomycetes, and fungi in the suspension were estimated. In general, the number of total bacteria and actinomycetes of the roots from the declining stand was larger, while the number of nitrogen-fixing bacteria was much smaller than healthy stand. Bacteria were randomly isolated from the agar plates, and the physiological characteristics of each isolates were examined. Bacterial flora from the healthy stand was diverse, while rather restricted groups were majorities in the isolates from the declining stand.
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