Auxins and inhibitors in the buds of
Pinus strobus were investigated. Auxins and inhibitors in ether extracts were measured by means of paper chromatography and Avena straght growth test.
On chromatographing in isopropanol-28%, ammonia-water(8:1:1), a growth promotingzone (Rf 0.0_??_0.5) and a growth inhibiting zone (Rf 0.5_??_0.9) were detected in the acid fraction and a inhibiting zone (Rf.0.3_??_0.9) was detected in the neutral fraction.
In the acid fraction, two auxins (Rf around 0.20, Rf around 0.25) and one inhibitor (Rf 0.54_??_0.84) showing positive reactions by EHRLIOH reagent and GORDON & WEBER reagent were found.
It may be suggested that they are indole compounds.
Chromatography reveals growth promoting substance which corresponds in Rf with IAA, while no color was observed upon application of EHRLIOH reagent etc. to the chromatogram.
The buds were treated with 1, 000 ppm solution of Tryptophane for 48 hours at 25C° in the dark.
After the treatment, auxins and inhibitors were measured.
Four auxins (Rf around 0.20, Rf around 0.25, Rf around 0.30, Rf around 0.41) andtwo inhibitors (Rf around 0.53, Rf 0.54_??_0.84) showing positive reactions by EBRLIOA reagent were found in the acid fraction.
Among them, auxin of Rf around 0.41 was identified as indoleacetic acid(IAA)comparing
it with Rf values in four solvents and color reactions in three reagents of synthesized IAA controls.
From these results, it may be considered that Tryptophane-IAA converting enzymesystem exist in the buds and these buds produce IAA from Tryptophane even without the addition of Tryptophane.
It may be suggested that the quantity of IAA obtained from the untreated buds might be too small to show the positive reaction by EHRLICH reagent etc.
The relation of the new compound (Rf around 0.30, Rf around 0.53) to Tryptophane was not established.
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