The in vitro properties of washed platelets (wPLTs) suspended in BRS-A additive solution which were prepared from one- or two-day-old platelet concentrate (PC) with an automated cell processor (ACP215) were studied during 72-hour storage.
Rate of plasma protein removal and platelet recovery by washing of PC were more than 98% and 87%, respectively.
When preparation was from one-day-old PC, pH was maintained above 6.8 during 72-hour storage. E800/E0, which is a parameter reflecting platelet shape, and MPV values increased and %HSR values were decreased by 10% immediately after washing, and then returned to pre-wash levels in a time-dependent manner. CD62P values were increased up to 36.8% by washing, but after that these values did not significantly change during 72-hour storage. Although swirling of wPLTs resulted in cloudiness immediately after washing, the solution became clear a couple of hours later. Similar results were obtained with wPLTs prepared from two-day-old PC.
These results indicate that the process of washing PC with ACP215 did not markedly degrade the in vitro properties of wPLTs.
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