Controlling animal epidermal diseases by applying aqua oxidized water has been assessed in animal clinical tests. Aqua oxidized water is known to posses a wide spec-trum of activies which are capable of inhibiting microbial growth in vitro. We have also applied aqua oxidized water to dermatomycosis infected Trichophyton verrucosum in cattle and exudative epidermitis infected by Staphylococcus hyicus in pigs. These mi-crobes have shown resistance to several anibiotics which are usually effective against the diseases.
We find that the effect of aqua oxidized water on the various species of cattle is different, which correlates to the decreasing γ-GTP, GOT and Eos in blood. We also find aqua oxidized water is effective on exudative epidermitis in pigs. These results suggest that the aqua oxidized water is clinically to a greater degree when compared to antibiotics on epidermal diseases in test animals as in results found in vitro.

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The therapeutic efficacy of orbifloxacin (OBFX), a new injectable quinolone, on neonatal diarrhea in piglets aged 6 to 18 days was clinically evaluated (stool normali-zation) and bacteriologically (change of cell number of Escherichia coli in rectal feces).
Intramuscularly administered OBFX at a dose of 2.5 or 5mg/kg/B. W. for 2-5 days showed a clinical cure rate of 100% in 27 piglets with diarrhea and a bacteriological ef-ficacy rate of 92.6%. The efficacy of OBFX seemed to be unaffected by the co-existence of a rotavirus and/or a transmissible gastroenteritis (TGE) virus, which were detected in the rectal stool of 19 and 11 animals, respectively, by predosing examinations. The susceptibility test of the E. coli, which was isolated in this study (231 strains), revealed that no strain was resistant to OBFX (MIC range:0.05-0.78μg/ml) and what of the strains were resistant (MIC: >100μg/ml) to oxytetracycline, kanamycin and ampicillin. No further clinical side effects were observed.
These results indicated that OBFX is useful antibacterial agent for controlling neonatal diarrhea in piglets.

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A 5-year old Japanese Black female cattle was examined because of abdominal pain and anorexia. The rectal temprature was 39.5°C. A tympanic sound was found in the right paralumber fossa. During rectal palpation, abdominal organs could be palpable as a large baloon in the abdominal cavity. Therefore, a right flank lapalotomy was performed. A 20- to 25-cm incision was made in the right paralumbar fossa. The flank laparotomy revealed the distention of cecum and a disk-like colon and three masses of fatnecrose. The laparotomy was explored to remove the one of the three masses and to detect possible complications. The flank incision was closed in a routine manner. One of the three masses of fatnecrose, which was found to constricting the disk-like colon, was surgically removed with care and there was an indication of cecotomy and colotomy. Thereafter appetite steadily improved and the patient recovered. We carefully considered the course of the operation. We should make a 30- to 40-cm width incision in the paralumber fossa. A decision was needed on the choice of which fatnecrose mass should be removed and on the matter of removing it. The removed mass was attached to the disk-like colon remarkably, and if it was removed uncarefully, the colon may have ruptured. It is important to perform the operatiom very carefully.

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A treatment trial of cephapirin injection (KP-10) for lactating stage was done in 55 cattle with mastitis (76 tests) at South-west Kaminoyama area, Yamagata Prefecture. KP-10 was given intramammaly at a dose of 250mg to a test for 3 days (s.i.d). In con-trol, combination of benzylpenicillin and dihydrostreptomycin sulfate was used. Clinical findings before and after treatment, modifoed CMT and microbial culture were com-pared between treated and control groups. Based on the clinical findings, and numbers of bacteria and cell, 85.4 and 64.3% was judged as effectively treated in KP-10 and con-trol groups, respectivery (P<0.05). Basaed on the udder fingings, milk and modified CMT, 72.9 and 57.1% was effective in the two groups, respevtively. Based on the improvment in cell number by modified CMT (agglutination) was 97.9 and 75.0% in the two groups, respecivery (P<0.01). Comparing numbers of the causal bacteria at pre-and post in administration, 83.3 and 57.1% of the cattle became negative in both groups (P<0.02). KP-10 was effective in 89.5% of the cattle with Staphylococcus sp . and 75.0% of those with Streptocoochu sp., respectively. In control, effectivess was noted in 88.9% for Staphylococcus sp. and 62.5% for Streptococcus sp., respectively. The causal isolated from the milk at pre-administration were highly sennsitiive to KP-10, but some strains or variants were weakly sensitive to penicillin and streptomycin. These results cleary re-vealed that KP-10 was effective to lactating stage mastitis in cattle.

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The relations among blood metabolites, body condition acores (BCS) and feedingdiets were investigated to clarify the factors influencing milk components. The results ofherds with low milk fat (under 3.5%) and with low milk solids-not-fat (SNF) (under8.5%) were compared with that of herds with sufficient milk components. In the low milk fat herds, the BCS in the dry period and total cholesterol levels in the middle andlate lactating stages were lower. In the low milk SNF herds, the BCS in the middle lactating stage, the plasma glucose level in the early lactating stage and the total cholesterol levels in the middle and late lactating stages were lower. Individual varieties ofprotein metabolites such as total protein, albumin and hematocrit were observed in boththe low milk fat and low milk SNP herds. These results show a low BCS in the dry period and low lipid intakes in the lactating stages of the low milk fat herds, and a lowenergy level in the lactating stages of the low milk SNF herds.

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A heterosexual twin female in cattle which showed the vital signs of being in heat at 10 month old, had a sex chromosomal chimerism (XX/XY). By embryo transfer, this heifer was fertilized and gave birth to a stillborn calf at full term. From these results obtained, this case was certified as a " fertile bovine heterosexual twin female with sex chromosomal chimerism (XX/XY) " . It seems that a choriovascular anastomosis in the fetal membrane of the developing heterosexual fetuses occurred after a critical point of differentiation of fetal ovary.

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[in Japanese]

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