Aqua oxidizing water (AOW) on pyoderma caused by Staphylococcus intermedius (S.intermedius) as well as Staphylococcus hyicus (S.hyicus) showed great therapeutic effects in piglets. Although the pathogenicity of S.intermedius was relatively small compared to Staphylococcus aureus and S, hyicus, it apparently brought about substantial growth hindrance and a complication due to the considerable physical stress by the pathogenicity. The pigs infected in pyoderma became weak due to menge mites at the beginning. Administering Ivermectin to the pigs appeared to be effective in treating the mites, however, allergy and pyoderma became worse. The weight of the heavily infected pig, dropped under 4kg, which was the pig's weight during the weaning period. We observed a tendency of recovery by spaying 300ml of the AOW for 3 days. After two weeks of treating the pigs with AOW, they totally recovered from pyoderma.

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The cutaneous horn on the white spot of the skin in Holstein dairy cattle.
Sadayuki Higuchi*, S. Horigome, G. Tsunoda1), H. Nemoto1) (Kennan Veterinary Clinic of Fukushima prefecture, 1)Fukushima Veterinary clinical & Technical Center)
Nail like phyma was admitted to the Holstein milking cow (about 3-4 years old). The diseased part was composed of two nail like phyma (10×15cm, 2-3cm in high) and many liner phyma (several mm in high) on the white spot of the skin. There were no worthy changes in the clinical symptoms or the blood. The proliferation of eoshinophic horny tissue and flat cells were admitted in phyma on histological examination. The thick epidermis and hyperkeratosis were admitted in the skin near phyma. There was remarkable proliferation of prickle cells in the thick epidermis. We suspected that the phyma in this case was caused by hyperkeratosis of the epidermis. It proved that phyma in this case was like a cutaneous horn. The cause of this phyma was not clear, but the influence of a ray causing solar keratosis was suggested as causing the original disease by phyma only on the white spot of the skin.

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To study the effects of the 1/29 Robertsonian translocation upon fertility in the herd of the Japanese Black Cattle breed, the cytogenetic survey and the examination of breeding data were carried out on daughters of a 1/29 translocation heterozygous sire. The cytogenetic survey was carried out on 146 daughters (a toral of 58 heifers which were composed of 31 repeatbreeder heifers and 27 heifers with normal fertility, and 88 cows) of this sire. Twenty-seven cases (87.1%) out of 31 repeat-breeder heifers showed the 1/29 translocation. On the other hand, fifteen cases (55.6%) out of 27 heifers with normal fertility showed the 1/ 29 translocation. When comparing the numbers with 1/29 translocation between repeat-breeder heifers and heifers with normal ferrility, significant differences (p<0.05) were statistically found. Thirty-nine cases (44.3%) out of 88 cows were heterozygous for the 1/29 translocation. When comparing the numbers with 1/29 translocation between 58 heifers and 88 cows, significant differences (p<0.01) were statistically found. The breeding deta of 34 daughter cows with 1/29 translocation and 40 daughter cows with normal karyotype from translocation sire, and 128 control cows were collected from the breeding records, and the data were compared among 3 groups, respectively. There was an increase in the number of inseminations per pregnancy and a decrease in the conception rate in the cows from 1/29 teanslocation heterozygous sire. From these results, it was suggested that the 1/29 translocation would affect the decrease in fertility, and would result in the increase in the culling rate which was caused by sterility in the heifers of Japanese Black Cattle. Also, it was surmised that the high culling rate in subfertile heifers with 1/29 translocation would lead to the decrease in the incidence of translocation (from 72.4% to 44.3%) in muliparous cows. Therefore, it seems that there is no correlation between the occurrence of 1/29 translocation and the breeding data in multioarous cows.

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To study the relationship between Robertsonian translocation and fertility in the herd of Japanese Black bulls, the cytogenetical survey was carried out on the 119 bulls. Ten bulls (8.4%) showed the 2 types of Robertsonian translocation; 1/29 translocation (6 bulls) and 7/21 translocation (4 bulls). Translocation carrier bulls were derived from 3 strains (C-E) out of 5 strains (A-E) in Japan, showing 1/29 translocation only in C strain, both types of translocation in D strain, and 7/21 translocation only in E strain. The semen quality in a bull with 1/29 translocation did not differ from those in the 4 bulls with normal karyotype. The conception rate (44.1% in average) in bulls with translocation was significantly different from those (62.5% in average) in normal bulls (p<0.05). As a result of the sperm chromosome study using an interspecific in vitro fertilization system with zona-free hamster oocytes, the incidence of chromosomally unbalanced spermatozoa was 4.4% (5/114 sperms) in a translocation carrier bull. The incidences of structurally abnormal spermatozoa were 29.8% (34/114 sperms) in a translocation carrier bull, and were higher than those of 15.2% (22/144 sperms) in normal bulls with a significant difference (p<0.01). From the results obtained, it seemed that 2 types of Robertsonian translocation are widely spread in the herd of Japanese Black bulls and may be derived from the fixed strains. And it was estimated that the cause of low fertility in translocation carrier bulls may be correlated with a high inci-dence of unbalanced and structurally abnormal sperms.

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The effects of acupuncture on uterine involution were investigated in dairy cows. The cows had been radiated by the CO2 laser to their acupuncture point (Jiao chao-chue). Twenty-seven Holstein cows were used, of which 22 cows radiated by laser as an experimental group, and 5 cows were not as the control group. The laser was radiated at 100 V. for 60 seconds, twice in all, every other day, 2 to 3 days after the parturition.
The amount of time required for the elimination of the lochia was 31.4 days on the average after the parturition for the control, while it was 21.3 days for the experimental group. The experimental group was eliminated earlier than the control, there being a significant difference (p<0.05). The average number of days necessary for complete uterine involution was 32.8 days for the control and 23.3 days for the experimental group, indicating the earlier involution in the experimental group (p<0.01). The days which elapsed between the parturition and the first postpartum ovulation were, on the average, 30.6 and 34.2 days for the control and experimental groups, respectively, although there was no significant difference in the days between the two groups.
These results suggest that laser acupuncture after the parturition has the effect of promoting uterine involution.

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