The insertion method for Houhai acupuncture point being effective on the rate of pregnancy has not been clearly verified yet even today. For this reason, we have tried unravel the insertion method for Houhai scientifically.
Cows in postparturient anaphrodisia was grouped into two groups, the long needle group (LN) and the short needle group (SN). The LN was tested to insert the needle from the hollow part located between the anus and the base of tail toward Kaifun to the apexossis sacri. The SN was tested to insert the needle 3 cm deep at the same angle and position.
The LN and the SN showed the period of estrus for 22 days and 66 days on an average, respectively. There was significant difference between these two groups. The average pe-riod of pregnancy and the average number of fertilization in the LN and the SN were 46 days and 1.3 times, and 56days and 2 times, respectively. There were no significant dif-ference beween two groups on the above. On the performance of pregnancy in the LN, 8 cows in 13 cows got pregnancy which occured with 88% per one insertion. On the other hand, 2 cows in 7 cows in the SN got pregnancy which occured with 50% per one inser-tion.

View full abstract

The factors effected for recovery of postpartum ovarian function were investigated from blood components during periparturition for 2 groups of Japanese Black cows which were divided into cows conceived by Al within 80 days after parturition (Group I) and cows not showing estrus within 80 days after parturition (Group II ). Blood samples were taken day 21 before parturition (day 0), and day 1, 5, 10, after then 10 every day until 90 days and the concentration of progesterone (P), BUN, Alb, NEFA, T-C, glucose, β-C, V.E were measured. The P levels in some case of Group I elevated day 30 after parturition. But all cases of Group II did not elevate untill day 90. There were not significantly different among 5 levels of BUN, Alb, NAFA, T-C, glucose, β-C in both groups. There were significantly higher (p<0.01) in serum V. E levels of Group I before parturition than those of Group II. From results obtained, it seemed that both groups were under the proper breeding conditions, because of the similar levels in NEFA and T-C to normal cows. On the other hand, it is suggested that the levels of serum V. E before parturition can make important factor for recovery of ovarian function, in addition to nutritional conditions.

View full abstract

By using aqua oxidizing water (AOW), we studied the comparison of germicide effect of the AOW and general washing and disinfection method in a bulk tank for milk. A number of bacteria decreased 250, 000 cfu/ml in the AOW whereas the general washing method was 50, 000 cfu/ml, which the AOW showed good result of the germicide effect.
To find the washing capacity and the effect of disinfection in a milker on the AOW, we diluted the milk with mastitis with the AOW in the diluting proportion of 1:1, 1:2, 1:4, 1:6, 1:8, 1:10, 1:20, 1:36, 1:40, 1:50, 1:100 and then, count the colony of becteria in the milk. We also mesured pH, redox potential and chlorine concentration in the above respective diluting mixtures. As the proportion of the AOW in the milk increased, pH and redox potential in tha AOW approched the initial mesurament. The chlorine concentration was found 0.7ppm in the diluting proportion of 1:36. The number of bacteria tended to be decreased over 1:10 proportion and bacteria did not found over 1:36 proportion of the mixture. Therefore, we found the germicide effect of the AOW was related to the chlorine concentration in the milk. In addition to this, although the improvement of milking hygiene is inevitable for lowering the initial bacterial number, washing the bulk tank right after the collection of milk was the most efficient method for decreasing the number of bacteria. The practical use of the AOW was considered that it is applicable to the improvement of the milk suction hygiene and the other wide fields.

View full abstract

Pre-dipping has not widely spread yet in Japan. This situation may be resulted from worry about iodine residues in milk when iodophor teat dip preparation is applied for pre-dipping. And any teat dipping preparations have not been approved for use of pre-dipping until Dec. 1997. In this study, changes of iodide ion concentration in milk was examined when post-dipping or pre-and post-dipping were carried out using low concentrated (0.1%) iodophor teat dip. In result, significant elevation of iodine level it milk was observed in both cases of post-dipping alone and pre-and post-dipping as 16.7 μg/l and 28.1 μg/l respectively. But there was no significant difference of iodin level between post-dipping and pre-and post-dipping group.
It was concluded that rising of iodine level in milk by use of 0.1% iodophor teat dip can not make health problem of milk in consideration of iodine level of the other com-mon foods.

View full abstract

To treat the reproductive disorders in cows, the authors used the Controlled Internal Drug Release (CIDR) device and investigated the induction rate of estrus and conception rate. By using CIDR, the estrus was induced in 96 (80.7%) of 119 cows and 77 (80.2%) of 96 cows showing the estrus were conceived by one time of Al and ET. The mean day from removal of CIDR to the estrus and Al was 2.4 days. There were no difference in the induction rate of estrus and conception rate among cows with ovarian quiescence, endometritis and silent heat that were diagnosed by the findings of ovaries and the open days after parturition. But the conception rate (60%) of cows with ovarian cyst was lower than those of other cows. From results obtained, the application of CIDR was effective for the treatment of reproductive disorders, and also an abbreviation of re-productive management in cows.

View full abstract

Abstract. A 24 month old Japanese Black heifer raised at a breeding farm was affected with lead poisoning and euthanized. Blood samples showed high levels of GOT and CK. Pathologically, pieces of lead-like metal were observed in the reticulum and ulcers were seen in the alimentary canal. Lead concentration of wet tissue in the liver and kidneys were detected at high levels by biochemical examination. It is suspected a fishing net rope containing lead wire which had been used to tie the heifer is the cause.

View full abstract