Physiopathological condition of ovarian cyst in cow was analysed by clinico-endocrinological, biochemical and histopathological investigations, and following results were obtained. (1)It was demonstrated by ultrasonographic observation that ovarian cyst(s) degenerated, never being persistent, and newly growing follicle(s) developed to new ovarian cyst(s) without ovulation, showing periodic vicissitudes with about 21 days cycle. (2)Some cows with ovarian cyst manifested the standing estrus at the time when the follicle(s) grew and developed to cyst(s). (3)Although blood progesterone level offered in low and estradiol-17β level increased to high during the stage where the cyst(s) degenerated and follicle(s) developed to new cyst(s), luteinizing hormone (LH) surge never occurred. (4)As to the no arise of LH surge, functional abnormality of the hypothalamic cyclic center was suspected. (5)The cow with ovarian cyst was considered to be chronic disturbance of the liver function and in oxidative stress, and such abnormalities might be related to the occurrence of ovarian cyst by obstructing the LH surge. (6)Remarkable degenerative changes occurred in granulosa cells of ovarian cyst reached maximum size. Detachment and fall of the granulosa cells took place and theca folliculi (theca interna and externa) became thin at the degenerating stage of the cyst.

View full abstract

The present studies were carried out to examine the efficiency of 2 drugs (PMSG and FSH) on the basis of the number of recovered eggs, fertilized eggs and normal embryos in the same cows from a single farm. The endocrine aspects were also examined to control the efficiency of superovulation by PMSG. At last, a new trial was made as an additional administration of gonadotropin releasing hormone analog (GnRH A) after PMSG and PGF2 α injections for the improvement of superovulation in cattle. (1) Individual cows showed differences in the ovarian response by two drugs, and did not show significant differences in the embryo collection following successful superovulaton. It seemed to be necessary to examine the endocrine aspects of cows during experiment to gain the efficient results of superovlation by PMSG. (2) It was obvious that cows showing the significant increases in E2 level from PMSG treatment to the estrus gave more numerous ovulatory follicles and normal embryos recovered. The increases in E2 levels from ovulation to embryo collection seemed not to have an injurious influence upon the results of embryo collection. (3) An additional administration of GnRH · A after PMSG treatment followed by a single insemination at a fixed time makes it possible to recover many normal embryos, and seems to be applicable to induce effective superovulation in the cows.

View full abstract

The 4 cases of urachal insufficiency in Japanese Black male. or castrated male cattle were admitted. The age of the cases is from 4 to 11-month-old. The growth rate of the cases were poor excepting case 2 . The body weight was 100 to 270kg. Physical examination revealed normal or decreased rectal. temperature, depression, anorexia, disuria or polyurea, and bilateral abdominal distention. The urinary bladder could not be identified on palpation per rectum excepting the case 2. Labolatory data included azotemia excepting the case 4. Laparotomy revealed large persistent urachus that had ruptured (case1), ruptured bladder and urachus that had one urolith (case2), obstuction of uretha and ruptured urachus (case3), and persistent urachus (case4). The urachus was removed surgically, but the all cases died or were slaughtered 3 to 65 days after surgery becuase of azotemia, renal calculosis, or unfavorable prognosis.

View full abstract