Buccal mucosa carcinoma-derived cell line, HO-1-N-1, epithelial-like cells, was obtained in order to investigate the characteristics of oral cancer cells and examine the [Ca
2+] i responses to stimulants, such as bradykinin (BK), histamine (HIST), thapsigargin (TG), epidermal growth factor (EGF) and transforming growth factor α (TGF α). Intracellular Ca
2+ influx was observed by all stimulants that enhanced the [Ca
2+] i response. However, intracellular Ca
2+ release was not observed in response to growth factors. The [Ca
2+] i response of BK (100 nM) was inhibited by 10 μ M of the BKB
2 antagonist, D-Arg- [Hyp
3, Thi
5, 8, D-Phe
7] -BK, and HIST (1 mM) was completely inhibited by 100nM of the H
1 antagonist, (+) -chlorpheniramine, in the presence and absence of extracellular Ca
2+ (1.5mM).A retrospective evaluation of the reticular form of oral lichen planus was made on the basis of clinical and histopathological observations in 55 patients. Patients were re-examined once every six months over a 25-year period. Biopsies were taken from all lesions. No cases of complete remission and no cases of malignant transformation were recorded. Our findings suggest that the reticular form of oral lichen planus is not a precancerous lesion.
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