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The Takahashi strain (“street virus”) isolated by mouse and rabbit brains from a patient, who died of rabies after a prolonged incubation period, has been passaged intracerebrally through mice and rabbits respectively. It seems that the Takahashi strain has been converted into fixed virus after 24 passages through rabbits, and 44 passages through mice, from the viewpoint concerning the shortening of incubation period, the change of symptoms, etc. However, considering the response of rabbits to the intracerebral inoculation of the mouse-brain-passaged virus and the irregularity of “negrigeness”, it would be concluded that the Takahashi strain has been changed to fixed virus after about 70 passages through mice.
During the experiments of the Takahashi strain in mice a variant strain (Taka-men) was obtained unexpectedly. This strain was isolated from the brain of one of 30 mice, which had been intraperitoneally injected with the mouse brain suspension of the 4th passages 2 times at a 4day interval. The Taka-men strain differs from the Takahashi strain distinctly in the short incubation period in rabbits and mice, the high intracerebral infectivity in mice, and the absence of subcutaneous infectivity in rabbits. Although these characteristics seem to show a tendency to fixation, Negri bodies have been found in the brain of mice and rabbits from the first to 54 and 13 passages respectively, disappearing thereafter. It is to be considered that, therefore, the Taka-men strain seems to be a variant strain of fixed type drivel abruptly from the original Takahashi strain, and that it varied to fixed virus competely after several passages. Such variationphenomenon of rabies virus induced by the intraperitoneal injection in mice is quite unusual, and I cannot find a report on the similar phenomenon.
As above mentioned, I have demonstrated from one strain (Takahashi) of street virus two series of variant. strains; the one has varied to fixed virus gradually, the other has abruptly acquired the properties of fixed type of virus, being converted into fixed virus completely through a few passages.

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In 1949 and 1950 I studied on the complement fixation test against Japanese B encephalitis on human sera; the sera of patients of Japanese B encephalitis and normal human sera, in Kyoto Prefecture, Osaka Prefecture (Takatsuki City) and Shiga Prefecture (Otsu City).
In this report I want to describe the results obtained.

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In this report, some foundamental aspects of available use of developing egg for study the experimental chemotherapy of virus were discussed.
Various dyestuffs, sulfonamides, antibiotics, two pyrimidin derivatives and five haloacylamid derivatives were tested for their antiviral action of vaccinia virus. Some of dyestuffs were effective by means of direct inactivation, but non to them inhibited their multiplication.

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In the course of our investigation about ectromelia virus which began the year before last, we happen to percieve the appearance of intraplasmic inclusion bodies to a great extent in monocytes which were always recognizable in ascites by intraperitoneal inoculation of the virus emulsion. Thus, we were enable to investigate inclusion bodies in free single cells under supravital conditions entirely free from various artificial forces which had previous been used to gather cells in which were found inclusion bodies. So, we would like to report concerning inclusion bodies in monocytes in ascites which have been the special subject of our observation, as we have made use of such techniques as Giemsa's staining, and simple staining, dark field microscopy and ultra-violet photomicrography in supravital conditions. Quantitative and qualitative developement of inclusion bodies and cell reaction appear in the periphery zone of the cytoplasm having completely on reaction with centrosphere of monocytes, and increase their volume gradually. In supravital state the inclusion bodies stained positive with neutral red and fuchsin but negative with nilblue, brilliantcresylblue and bordeaux BX. Elementary bodies within the inclusion bodies having blue brilliant points were found in the supravital preparations by the dark ground microscope. It was possible to take ultraviolet photomicrographs of the infected monocytes in the supravital preparations. From data obtained by this method we observed the fine figures of elementary bodies in the ectromelia inclusion bodies corresponding to the blue points which had been found by the dark gorund microscope. Absorption of ultraviolet rays by inclusion bodies is much less than by the nucleis, and there is no difference between that of the inclusion bodies and that of the protoplasm. The results got from the ultraviolet microskopy coincide with the other results that the inclusion bodies give a negative Feulgen test in smear preparations.

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Adsorption states of influenza virus, Lee strain, on chicken red cells were studied under electron microscope. Treating electronmicrographs quantitatively, adsorption end point was determined. From this end point, that the foundamental idea of CCA titration by Miller and Stanley had been fare, was proved. From some pictures, it was concluded that owing to agglutination of virus particles by antisera, the red cell agglutination inhibiton reaction would be caused. And virus particles adsorbed on chicken red cells were inversely eluted and agglutinated when anti-viral serum added after complete adsorption. This latter phenomenon was named as “robbing off reaction” by antisera.

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