Yolk sac of chicken eggs infected with Rickettsia mooseri was cultivated, and the rickettsiae in the grown cells were observed with light- and electron microscopes.

抄録全体を表示

Affinity of the rickettsiae to tissue cells, especially the relation between rickettsiae and host cells has been studied.
Rickettsiae infected tissues of embryonated chicken eggs were cultivated, and observations were made on the growth cells. On the other hand, normal tissues of the same kinds were cultivated side to the infected tissues, and susceptibility of each tissue to the infection was examined
Epithelial cells were found to be more susceptible to the infection than fribroblasts.

抄録全体を表示

(1) 小麦黄色モザイク病 (縞萎縮病) ヴィールスによる封入体 (X体及び結晶性封入体) の各種染色反応並に細胞化学的検索を行つた.
(2) 本X体と寄主細胞核とはTrypan blue, Erythrosin-Trypan blue, Erythrosin-Methylen blue, Pyronin-Methyl green, Giemsa各染色で明らかに染め分けられる.
(3) 塩酸・トリクロル醋酸・過塩素酸で核酸除去の操作後, Trypan blue, Giemsa, Pyronin-Methyl green各染色を行つた結果, X体はリボ核酸 (RNA) を持つことが想像された.
(4) X体は塩基性色素で甚だしいメタクロマジーを起さず, 結晶では二・三色素でメタクロマジーが見られた.
(5) X体は蛋白反応陽性, 特にTyrosine・Arginineの存在が確認されたが, Tryptophane・Indol系物質は明らかでなかつた.
(6) X体はSudan IIIで染り, リポイドを有する. Feulgen反応は陰性.
(7) 被害細胞内並にX体中の空胞内に, 蛋白反応を呈しリポイド反応陰性の, X体と同じ染色反応を示す針状結晶が認められた.
(8) 以上の事実から結晶は所謂paracrystalに属し, X体より濃度の高いヴィールス性物質と考えられる. 又X体は内部にヴイールスを有する, 寄主細胞質特にミクロゾームのヴィールス侵入に対する一種の防禦反応生成物と推定された.
終りに臨み本研究に終始御懇篤な御指導を賜つた盛岡試験地主任八柳技官に深謝する

抄録全体を表示

The onset of eastern equine encephalomy elitis and western equine encephalomyelitis can be observed following the intratesticular inoculation of the viruses in mice. The virus content of each organ (blood, spleen, testis, brain and spinal cord) of the inoculated mice was observed periodically by means of intracerebral inoculation of mice.
The obtained results show that the viruses inoculated intratesticularly enter the blood circulation immediately and then propagate rapidly in the central nervous system. Following the virus multiplication in the central nervous system, the intratesticular multiplication was also recognized.

抄録全体を表示

Measles virus, which derived from patients and was attenuated by intratesticular passages through guinea pigs, was inoculated into susceptible children by subcutaneous injection or intranasal spraying, Clininical and immunological findings observed were as follows:
1) A mild fever as well as change apparently the Koplik spot were found only in one case of ten, but neither katarrhal signs such as cough, sneeze or conjuctivitis nor eruptions were evident.
2) Of the blood picture, specific findings were: Initial leucocytosis followed by the leucopenia, relative neutropoilia and lymphopenia, as well as slight monocytosis and eoslnopenia. These changes were similar to those induces by the natural infection of meales.
3) It was deemed likely that the meales virus muliplied and the neutralizing antibodies were produced in the bodies of inoculated humans.

抄録全体を表示

We tired to prevent measles with attenuated measles virus, which was obtained from patients and weakend by intratesticular passage through guinea pigs.
The immunization was made by intranasal spraying for susceptible children.
The results obtained were as follows:
Classification of response Number Control
With evidence 130 (33.8%) 156 (59.%)
typical 12
mild 81
minimal 31
no count 6
No evidence 256 (66.2%) 106 (40.5%)
with chance to be infected
from patients with measles 111
no chance to be infected
from patients with measles 85
not clear 60
total 365 262
A-significicant differece could be found between the results of the prevented and control groups.

抄録全体を表示

ヴィールス学の進歩と共に多数のヴィールスが分離され各々の牲状が詳細に究められるにつれて, ヴィールスの分類という問題が当然起つてくる. この点に関連して, インブルエンザ・ヴィールスの分離者として名高いイギリス国立衛生研究所C. H. Andrewes博士から世界各地のヴィールス学者に質問状が発せられた. 我国に対しては予防衛生研究所小島三郎博士の許にそれが届けられ, 同博士から折返えし回答がなされた. その内容は専らインフルエンザ群ヴィールスに関するものであるが, 基本的趣旨は広くヴィールス全般に係るものであり, 多くの問題をはらんでいると考えられるので, 小島博士の御諒解を得て全文を掲載することとした.

抄録全体を表示