Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
ISSN-L : 1342-4580
Volume 19 , Issue 3
Showing 1-10 articles out of 10 articles from the selected issue
Original Paper
  • Yogeeta ANAND, Y. K. BANSAL
    Type: Original Paper
    2002 Volume 19 Issue 3 Pages 159-162
    Published: 2002
    Released: April 25, 2005
    JOURNALS FREE ACCESS
    Shoot buds of Adhatoda vasica Nees. isolated from multiple shoot cultures were encapsulated in 3% sodium-alginate with different gel matrices. Maximum conversion of the encapsulated shoot buds into plantlets was achieved on Gamborg’s B5 medium containing 4.65μM Kinetin and 50mgl-1 Phloroglucinol and the plantlets were successfully grown in soil.
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  • Doddapaneni HARSHAVARDHAN, Thammiraju Shyamala RANI, Kandasamy ULAGANA ...
    Type: Original Paper
    2002 Volume 19 Issue 3 Pages 163-171
    Published: 2002
    Released: April 25, 2005
    JOURNALS FREE ACCESS
    An improved and producible protocol for in vitro regeneration of sorghum [Sorghum bicolor (L.) Moench] from shoot meristem explant is reported. By striking an optimal balance between a weak auxin like naphthalene acetic acid (NAA) and the cytokinin thidiazuron (TDZ), the isolated shoot meristems were manipulated to follow either organogenic or embryogenic pathway. There was no intermediate callus formation. Multiple buds were induced on enlarged meristems using MS medium with 5.0μM of TDZ, 17.72μM Benzylaminopurine (BAP), and 1.074μM NAA. To maximize the number of bud initials per explant, three parameters (seed size, germination technique, and age of explant) were studied. Five to 7-day-old shoot meristems responded best with ≥80% induction of bud initials. Seed size was not significant in influencing induction potential of the shoot meristems. Six weeks after in vitro culture, each meristem produced 35-40 shoot buds. Direct somatic embryogenesis (≥80% induction) was accomplished following a two-step culture procedure consisting of induction of multiple buds and formation of somatic embryos. A high frequency (700-1000) of somatic embryos per explant was obtained on MS medium with 17.72μM BAP and 2.69 μM NAA. Shoots from both organogenic and embryogenic pathways rooted on half-strength Murashige and Skoog (MS) medium with 8.28μM Indole 3-butyric acid (IBA) and 1.14μM Indole acetic acid (IAA). After one month on rooting medium, plants with well-developed roots were transferred to jiffy cups. Such plants were subsequently acclimatized in the glasshouse, and were grown till maturity; they showed normal seed set. Random amplified polymorphic DNA (RAPD) analysis of regenerants did not detect any DNA polymorphism.
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  • Lanzhuang CHEN, Reiji OKABE, Takuro HAMAGUCHI, Liming GUAN, Taiji ADAC ...
    Type: Original Paper
    2002 Volume 19 Issue 3 Pages 173-179
    Published: 2002
    Released: April 25, 2005
    JOURNALS FREE ACCESS
    Cultures were made of ovaries guineagrass (Panicum maximum) harvested in different seasons to obtain plant regeneration via somatic embryogenesis. Callus formation and plant regeneration occurred when the ovaries harvested 0-7 days after anthesis (DAA) in summer were cultured on Murashige and Skoog medium with suitable hormones. However, most of the ovaries harvested in winter did not form calli when cultured similarly. To determine the reason for this difference, flowers at 0-7 DAA were collected during both seasons and observed under Nomarski differential interference-contrast optics. The ovaries collected at 0-7 DAA in summer showed normal development and matured seed set-up. In contrast, most of the ovaries collected at 0-7 DAA in winter degenerated gradually. This result suggests that embryo-sac analysis of plant materials provides important information on whether plant materials are suitable for the induction of calli.
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  • Hiroshi TAKAZAWA, Kouichi YOSHIMURA, Akira IKUTA, Kiichiro KAWAGUCHI
    Type: Original Paper
    2002 Volume 19 Issue 3 Pages 181-186
    Published: 2002
    Released: April 25, 2005
    JOURNALS FREE ACCESS
    Callus tissue from the stems of Actinidia arguta (Actinidiaceae) produced the following eight ursane-type triterpenes: α-amyrin; uvaol; ursolaldehyde; ursolic acid; corosolic acid; asiatic acid; 2α, 3β, 24-trihydroxyurs-12-en-28-oic acid (4-epiasiatic acid); and 2α, 3α, 24-trihydroxyurs-12-en-28-oic acid. The tissue also produced three oleanane-type triterpenes (β-amyrin, oleanolic acid, and maslinic acid), and two phytosterols mixtures (sitosterol and stigmasterol). Seven of the eight ursane-type triterpenes (the exception being asiatic acid), the three oleanane-type triterpenes, and the two phytosterols mixtures were also isolated from A. chinensis and A. polygama callus tissues. Variations in the production ratios of two of the triterpenes (ursolic acid and oleanolic acid) and the two phytosterols mixtures (sitosterol and stigmasterol) were compared among callus tissues from the three plant species.
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