One of the major differences between fibrinogen degradation products (FgDP) and fibrin degradation products (FDP) exists in the fact that D-dimer can be proouced in the process of late FDP as a results of crosslinkage of γ-γ chains of fibrin and only D-monomer is in late FgDP. Therefore, a new method for detection of D-monomer representing FgDP and D-dimer indicating FDP was described in this paper, and the two dimensional immunoelectrophoresis using SDS-agarose gel in the first dimension step and agarose gel containing anti-D or E serum in the second dimension step was applied for this new method.
D-monomer of late FgDP and D-dimer of late FDP were prepared and studied on our method with anti-D serum, showing characteristic electrophoretic-movilities of those two human materials. Also, from the plasma containing D-monomer or D-dimer the derivatives of FgDP and FDP could be differentiated by our method with anti-D serum.
On the other hand, the fibrinolytic lysates of a clot from a patient with factor XIII deficiency did not demonstrate D-dimer by the use of our method, but presented D-monomer. After the factor XIII concentrates were added into the patient plasma in the 10% final concentration of factor XIII, D-dimer was detectable in the fibrinolytic lysates. Furthermore, when this method was applied for a patient suffering from APL with DIC, no D-dimer was detected in the patient serum. Therefore, it might be suggested that the impairment on the formation of stabilized fibrin in a case of APL with DIC might play an important role as a cause of bleeding tendencies.
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