Collection of embryos from 47 cows, whose sexual cycle was confirmed to be nonnal, was made by perfuseing their uterine horn beside of corpus luteum by such non-surgical methods as automatic perfusion technique, 7-8 days after aftificial insemination during spontaneous estrus period. The percentage of success in collecting embryos and the collecting rate of transferable embroys was 77.6%(38/49), 61.2%(30/49) respectively.
The relationship among the period from the delivery to the collection of embryos the percentage of success in collecting embryos and the collecting rate of transferable embryos was also investigated according to the date on the 150th day after the delivery. Before the 150th day the percentage of success in collecting embryos and the collecting rate of transferable embryos were 100%(16/16) and 88%(14/16), while after the 150th day they became 67%(21/31) and 51%(16/31), respectively. These rate were significantly higher before the 150th day (px<0.05).
As the result of transfered 12 collecting embryos to 12 recepient Holstein cows, the high pregnancy rate 75%(9/12) was obtained.
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