It is a deniable fact that cytoplasma showsvarious picture by varying fixatives.
For the purpose of observing this effect, scan- ning electron microscie was used.
Materials are superficial and intermedate squamous cells of female genital tract, taken directlyfrom the cervix with an Ayres spatula.
These specimens were prepared with 3 typesdifferent fixatives each consist of following ingr- edient.
1) 1% glutaraldehyde. (standard)
2) 20% ethylalcohol.
3) 50% ethylalcohol.
Then dehydrated in aceton series, and coatedwith 200A thick layer of carbon and gold, exa- mined in a JSM-SI scanning electron micrscope.
The surface of cells appeared rough and cove- red by prominent network such as microridgesor micro villi like structures, but there is noevidence among different techniques of specimenpreparation.
One week later appeared small and irregularchanges on the surface of cytoplasmic rim.
This findings may be degeneration, and remarkable in specimen prepared with 20% alcohol.
Two weeks later, cytoplasmic changes progressed vastly whole surface showed degenerative findin- gs, but in specimen prepared with 50% alcohol, nuclear shape were barely distinguished.
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