To study the feasibility of determining the mean annual aboveground biomass gain of tropical seasonal forests at the national scale, we estimated the gain (i.e., increase due to growth of living trees) and loss (i.e., decrease due to tree death) of aboveground tree
stand
biomass (
stand
AGB) using 49 permanent sample plots distributed nationwide for 139 observation periods from 2005 to 2015 in Cambodia. In a linear mixed-effects model,
stand
AGB gain was predicted to increase with the initial
stand
AGB:
Stand
AGB gain=0.0165
Stand
AGB+2.20 (
n=139,
P<0.0001,
R2=0.4531, RMSE=2.84), where
Stand
AGB gain is the sum of tree AGB growth (Mg ha
-1 year
-1), and
Stand
AGB is the sum of initial tree AGBs (Mg ha
-1). The mean estimated
stand
AGB gain was 4.79Mg ha
-1 year
-1 for an average initial
stand
AGB of 155.5Mg ha
-1. The annual
stand
AGB loss was<20% of the initial
stand
AGB and the influence of
stand
AGB loss on
stand
AGB gain was negligible. The 2006 IPCC Guidelines for National Greenhouse Gas Inventories assigned
stand
-age-dependent values of default annual
stand
AGB gain for tropical natural forests. However, age is difficult to determine in tropical trees. Our
stand
-age-independent approach based on the
stand
AGB offers a practical method for assessing the AGB gain of tropical natural forests.
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