In order to study the effects of residual stenosis on myocardial salvage, we created % coronary stenosis with or without contrast washout delay at reperfusion in six groups of dogs. In Group A (n=), the artery was occluded for 1h before being fully reperfused. In Group (n=), the artery was occluded for 1h, then subjected to 6h of % stenosis without contrast washout delay. In Group C (n=), the artery was occluded for 1h, followed by 1 week of % stenosis without contrast washout delay. In Group D (n=10). again the artery was occluded for 1h, then subjected to 6h of % stenosis with contrast washout delay. In Group (n=). the artery was occluded for 7h, then fully reperfused for 1 week. Finally, in Group F (n=). the occlusion lasted for a full week. All dogs were sacrificed 1 week after occlusion. In Group A, myocardial creatine phosphokinase activity (CK) in the inner layer was 43.±12.5% that of non-infarcted myocardium. Myocardial CK in Group (46.5±7.4%) was little different but in Group C it dropped to 26.6±.4%, suggesting that % residual stenosis is not deleterious if it is continued for 6h or less but that it will result in considerable depletion of myocardial CK, if it is sustained for 1 week. In Group D, myocardial CK dropped markedly to 11.3±3.7%, little different from that for either Group (13.3±2.6%) or Group F (.3±3.3%). This suggests that contrast washout de-lay following % stenosis is not beneficial to myocardial salvage. Infarct size, as determined using triphenyl tetrazolium chloride as a stain was smallest for Group A (6.±3.6%) and largest for Group F (62.6±.%). Group showed smaller infarct size (13.2±3.7%) than did either Group D (27.±5.5%) or Group (25.3±6.3%). From this. we came to the conclusion that % residual stenosis without contrast washout delay is not deleterious provided it is sustained for 6h or less.

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樟芝菌糸体中の新規マレイン酸及び琥珀酸誘導体(antrodin A-)の定量分析法を確立する目的で，高速液体クロマトグラフィー(HPLC)による分析条件を検討した。Antrodin A-は，移動相：1 % AcOH/H₂O-CH₃CN( min, 60:40;40min, 30:70)；流速：1.ml/min；測定波長：235 nm；カラム温度：室温の条件下，ODSカラムを用い定量を行った。この方法による日内と日間の相対的な標準偏差は4.6%及び4.1%(n=5)であり，.1(or .2)μgから1.2μgまでの濃度範囲で良好な直線性が得られた。Antrodin A-の回収率はそれぞれ., 102., 101., 100.5及び.%であった。本測定法は迅速且つ正確であり，樟芝菌糸体中の新規マレイン酸及び琥珀酸誘導体の定量に適していると考えられる。

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性周期における牛の末梢血中遊離estrogen測定にITTRICH螢光法を応用して次の成績を得た。
Ittrich colorの最大波長をspectrofluorometer Hitachi MPF-2AおよびType203で測定した結果,励起光538nm,螢光552.5nmであった。この螢光特性は, およびにそれぞれ共通であった。実際の測定では最大波長が接近しているので感度は若干低下するが510~520nmで励起し•螢光側552•5±•5nmを読み,ALLENの補正を行なった。この条件において,および-methyletherの最少検出量は1ngであった。回収率補正の目的で加えた6,7-³H--17βの全過程における回収率は平均60.3±11.7%であった。正常性周期を示す黒毛和種2頭の頸静脈血についてestrogenを分画測定した。その結果,両牛共,の各消長型は性周期の全期間を通じてほぼ同じ傾向を示したが,はにくらべ全般に高値であった。または検出されなかった。これらの牛のtotalestrogenは発情前期に増加し,排卵前に鋭いピーク(35.3および/l;および/l,および/l)を形成し,排卵後は急激に減少して最低値(3.~5.3ng/l;および/l,および3.4ng/l)を示した。黄体期の最高値(10.1および/l;および3.4ng/l,および23.6ng/l)は排卵後6~日に認めた。すなわちestrogenの血中濃度は性周期の間に2つのピークを形成することを認めた。

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We conducted this study to clarify the relationship between manganese solubility and grain sizes of Mg-Al alloys. Mg-5%Al, Mg-%Al, and Mg-11%Al alloys were prepared using high-purity magnesium (>.%) and aluminum (.%). These alloys were melted at 1123K by adding electrolytic manganese (.%) and solidified as alloy ingots after continuous stirring for 21.6ks. The alloy ingots were then remelted at 933K, 998K and 1073K, and held statically at each of these temperatures for 21.6ks. By this process, the excess Mn that existed over its liquid solubility precipitated and sedimented to the bottom of the melt. Thus, the solubility of Mn in liquid Mg-Al alloys was determined by analyzing the upper surface of the ingots quenched from each holding temperature. Consequently, the following equations were derived as experimental formula for determining solubility of Mn in liquid Mg-Al alloys at different temperatures: Y = 1.79-6.×10^-2X (1073K), Y = 1.28-4.41×10^-2X (998K), and Y = .90-3.35×10^-2X (933K), where X is the concentration of Al (mass%) and Y is solubility of Mn (mass%). Mg-%Al alloys containing to 3% Mn were prepared in order to investigate the influence of Mn on grain sizes, and microscopic observations of these alloys were carried out with and without superheating of the specimens. The grain diameter of high-purity Mg-%Al alloys (% Mn) is approximately 40 μm, which is finer than that of the commercial AZ91 magnesium alloy with superheating. Therefore, high-purity Mg-%Al alloys have an essentially fine-grained structure. An increase in the Mn content tended to coarsen the grain structure of Mg-%Al alloys that contain .02 to 2.27% Mn; however, the coarse-grained structure can be refined by superheating. Superheating plays a role in resetting the coarse-grained structure to a high-purity structure (initially fine-grained) when Mn is added. Further, it is clarified that the solubility of Mn in liquid Mg-Al alloys exhibits no relationship with either the grain size or the superheating effect.

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We conducted this study to clarify the relationship between manganese solubility and grain sizes of Mg-Al alloys. Mg-5%Al, Mg-%Al, and Mg-11%Al alloys were prepared using high-purity magnesium (>.%) and aluminum (.%). These alloys were melted at 1123 K by adding electrolytic manganese (.%) and solidified as alloy ingots after continuous stirring for 21.6 ks. The alloy ingots were then remelted at 933, 998, and 1073 K, and held statically at each of these temperatures for 21.6 ks. By this process, the excess Mn that existed over its liquid solubility precipitated and sedimented to the bottom of the melt. Thus, the solubility of Mn in liquid Mg-Al alloys was determined by analyzing the upper surface of the ingots quenched from each holding temperature. Consequently, the following equations were derived as experimental formulae for determining solubility of Mn in liquid Mg-Al alloys at different temperatures: & Y=1.79-6.×10^-2X (1073 K),
& Y=1.28-4.41×10^-2X (998 K),
& and Y=.90-3.35×10^-2X (933 K), where X is the concentration of Al (mass%) and Y is solubility of Mn (mass%).
Mg-% Al alloys containing to 3% Mn were prepared in order to investigate the influence of Mn on grain sizes, and microscopic observations of these alloys were carried out with and without superheating of the specimens. The grain diameter of high-purity Mg-% Al alloys (% Mn) is approximately 40 μm, which is finer than that of the commercial AZ91 magnesium alloy with superheating. Therefore, high-purity Mg-% Al alloys have an essentially fine-grained structure. An increase in the Mn content tended to coarsen the grain structure of Mg-% Al alloys that contain .02 to 2.27% Mn; however, the coarse-grained structure can be refined by superheating. Superheating plays a role in resetting the coarse-grained structure to a high-purity structure (initially fine-grained) when Mn is added. Further, it is clarified that the solubility of Mn in liquid Mg-Al alloys exhibits no relationship with either the grain size or the superheating effect.

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The decay of 67h

99Mo

to the levels of

99Tc

has been studied with Ge(Li), Ge and Si(Li) detectors. Some new gamma rays of energies of 38, 163.4, 319.

8

, 321.

0

, 352.

9

, 599.6, 727.07 and 1082.2 keV have been observed. One new level at 163.4 keV is suggested and two controversial levels at energies 726.7 and 1081.4 keV have been confirmed. The directional correlation system has been used to measure the 740–181, 740–(40)–140, 961–181 and 822–(40)–140 keV cascades. These measurements confirm 3/2⁺, 5/2⁻ and 1/2⁻ spin assignments for 921.

0

, 1004.2 and 1142.1 keV levels. The mixing ratios of 40.585, 140.511, 739.7, 823.1 and 961.

0

keV gamma rays have been obtained as M1+≤6.25×10⁻²%

E

2, M1+

9

±1%

E

2,

E

2+

8

.

0

±1% M1,

E

1±1% M2 and M2+

E

2, respectively.

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The phase diagrams of the Ni-Mo- and Ni-W- ternary systems in the region of less than 50mol% were constructed by thermodynamic calculation, based on the data obtained by thermodynamic measurement of the related materials. We found three ternary eutectic points and three or two ternary peritecto-eutectic points as follows:
:L (1365K, 71.5mol%Ni-6.mol%Mo-.5mol%)=(Ni)++
:L (1355K, 62.5mol%Ni-2.5mol%Mo-30.5mol%)=++
:L (1445K, 42.mol%Ni-30.6mol%Mo-10.3mol%)=(Ni)+NiMo+
P₁:L (1812K, 34.mol%Ni-42.3mol%Mo-.mol%)+MoB=+
P₂:L (1633K, 42.3mol%Ni-40.4mol%Mo-17.3mol%)+Mo=+
P₃:L (1812K, 53.5mol%Ni-33.7mol%Mo-12.mol%)+Mo=NiMo+
:L (1622K, 51.mol%Ni-31.6mol%W-17.4mol%)=(Ni)+W+
:L (1260K, 71.mol%Ni-7.mol%W-.mol%)=(Ni)++
:L (1291K, 65.4mol%Ni-4.mol%W-29.mol%)=++
P₁:L (2115K, 23.mol%Ni-43.1mol%W-33.1mol%)+WB=+
P₂:L (1657K, 48.mol%Ni-33.1mol%W-18.mol%)+=W+
The calculated phase diagrams are expected to be useful for the development of new Ni-based heat-, corrosion- or wear-resistance alloys.

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Emblica officinalis (.O) extract was found to dose dependently lower the incidence of liver tumors induced by N-nitrosodiethylamine (NDEA). Control group rats (NDEA alone) had 100% tumor incidence, whereas in animals treated with .O at a dose of 250mg/kg body weight, the incidence was %. The liver weight of the control group was 7.±1.51g/100g body weight, and that of the .O-treated group showed a dose-dependent decrease (4.90-3.50g/100g body weight). NDEA treatment also increased serum and liver γ-glutamyl transpeptidase (γ-GTP) activity to 135.5±27.U/liter and 2.90±.08nmol/min/mg protein, respectively; and .O treatment reduced these levels in a dose-dependent manner (.-44.U/liter and 2.25-.38nmol/min/mg protein). The level of liver aniline hydroxylase, a P-450 enzyme, was increased by the NDEA treatment, and this increase was reduced by the administration of the .O extract. Liver glutathione-S-transferase (GST) activity, which was increased to 1, 414±137nmol/min/mg protein by NDEA treatment, was found to be reduced in a dose-dependent manner by .O in vivo. .O extract was also found to inhibit aminopyrene-N-demethylase activity in vitro, with 301μg/ml as the concentration needed for 50% inhibition. The extract was also found to inhibit DNA adduct formation induced by benzo(a)pyrene [(a)P] and aflatoxin 1 (AFB1). The concentration of .O extract required for 50% inhibition of microsome-catalyzed (a)P adduct formation was 280μg/ml; and that for AFB1 adduct formation, 120μg/ml. The results of the present study indicate that .O extract can interfere with the metabolism of NDEA and inhibit liver tumor development by this carcinogen, suggesting its use in the prevention of hepatocarcinogenesis.

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Cefotaxime (CTX) に高度耐性を示すEscherichia coli11株, Klebsiella 株の合計33株について, 産生されるβ-lactamaseに関する基礎的検討を実施した. CTX高度耐性のプラスミドは, .coliで/11株 (81.%), K. pneumoniaeで21/株 (95.5%) の合計30株において受容菌に伝達された. 接合伝達株の基質特異性は, benzylpenicillin (PCG) に対する相対加水分解率がCTX: 38～95%, ceftazidime (CAZ): ～.6%, aztreonam (AZT): ～56%とCTXが最も高かった. P/Cアーゼテストでは全株PCG分解菌と判明した. M1のプラスミド上に存在するCTX耐性遺伝子873bpのオープンリーディングフレームを含む領域をクローニングし, DNA塩基配列を解析した. その結果, アルゼンチンで分離されたSalmonella typhimuriumより検出されたCTX-M2型のExtend-Spectrumβ-Lactamase (ESBL) 遺伝子とDNA相同性が100%一致し, 国内で報告されているToho-1型とも.%の相同性を有することが明らかとなった. それを基にプライマーを設計しPCR法により遺伝子の検索を実施したところ, 33株全てにおいて同一プライマーで増幅される遺伝子の存在が認められた.

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This study aimed to determine the prevalence of fecal carriage of extended spectrum β-lactamase (ESBL) and/or plasmidic AmpC β-lactamase (pAmpC) producing Escherichia coli among dogs (n=428) in Turkey. Polymerase chain reaction (PCR) and sequencing were used to characterize genes encoding β-lactamase and plasmid mediated quinolone resistance (PMQR). Antimicrobial susceptibility testing and PCRs for virulence genes and phylogenetic groups were also performed. Cefotaxime resistant

. coli isolates were detected in 95 (.2%) of the swab samples. Sequencing analysis results showed occurrence of various β-lactamase genes: bla_CTX-M-15 (62), blaTEM-

1b

(42), bla_CMY-2 (

22

), bla_CTX-M-3 (16), bla_CTX-M-1 (15), bla_OXA-1 (

9

) and bla_SHV-12 (3) alone or in combination. The most frequently encountered phylogenetic group was group A₁ (35.

8

%), followed by group D₂ (

22

.1%),

B

1 (15.

8

%), D₁ (

9

.5%),

A₀

(7.4%),

B2₂

(5.3%) and

B2₃

(4.2%), respectively. PMQR genes, aac(6’)-Ib-cr, qnrS1 and qnrB10 were detected in 25.3, 10.5 and 1.1% of the isolates, respectively. While all isolates were susceptible to imipenem and amikacin, resistance rates to non-β-lactam antibiotics ranged from 20.

0

% for tobramycin to 56.

8

% for tetracycline. The virulence genes were only detected in 34 (36.2%) of the isolates and this isolates carried single or various combination of virulence genes of iucD, papC, papE, f17a-A and eaeA. Four isolates were identified as human virulent pandemic CTX-M-15 producing

E

. coli clone O25

b

:ST131/

B

2. To the best of our knowledge, this is the first study to show fecal carriage of ESBL/pAmpC type β-lactamase producing

E

. coli isolates among dogs in Turkey.

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千葉大学病院のホールボディカウンタを用いて, 405名の健常人と186名の患者を対象に, 全身カリウム (K) 量の測定を行った。全身K量は以下に示すレファレンス値として算出した。すなわち20歳代の健康人の全身K量を標準値とし, 体表面積で除し, かつ年齢と男女差を加味して補正し%表示した。405名の健常人のレファレンス値は100.65±.%であった。各疾患ごとのレファレンス値は以下のようになった。肝硬変: 94.24±11.%, 慢性肝炎: 95.74±11.24%, 甲状腺機能亢進症: .37±10.%, バーター症候群: 82.±.01%, 周期性四肢麻痺: 93.±.86%, 筋無力症: 97.34±6.42%, 低カリウム血症: 90.64±11.76%であった。また子宮癌, 乳癌, 貧血, 高血圧症ではそれぞれ, 97.78±11.5%, .±.88%, 96.64±12.73%, 98.5±.63%となった。14例が75%以下の異常低値を示した。その内訳は肝硬変1例, 高血圧症3例, 糖尿病1例, 低カリウム血症3例, 周期性四肢麻痺1例, バーター症候群2例, 薬物中毒2例, 乳癌1例であった。バーター症候群の1例, 周期性四肢麻痺の3例, 高血圧症2例, 肝硬変1例, 筋無力症1例では, 経時的に全身K量が測定され, 病状の経過との間に関連が見られた。

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Repetitive liquefactions in angular and subangular gravels from an alluvial fan, in the case of two earthquakes with accelerations lower than that of a former which had caused liquefaction some months before, are described. The liquefaction took place from the surface, down to a depth of approximately 10 m. Water laden with sediments spouted out for some tens of minutes after the strong motion ceased. Knowledge of the grain size distribution of the sediments inside the body of the alluvial fan roughly defines the areas of the fan prone to future liquefaction. Non-repetitive liquefaction in overconsolidated fine fluvio-lacustrine sand of glacial origin at the unusual depth of 14 m to 16 m is also described. From the observation of sand lifted up for 10 m inside a large diameter well, and of that which spouted out from several microvents, it was estimated that extra-pore pressure was close to lithostatic conditions. Nevertheless, modeling of intrinsic pore pressure buildup on the DESRA2 programme gives lower values than the experimental one. Geotechnical characteristics of the 12 m-thick sandy stratum indicate that the upper part is unusually soft, either due to water circulation or past repetitive liquefactions which prevented reconsolidation. It is suggested that excess pore pressure generated by the earthquake was also due to redistribution of pressure from adjacent sediments.

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 The stability of

‒tetrahydrocannabinol acetate (‒THC‒OAc) and ‒tetrahydrocannabinol acetate (‒THC‒OAc) in -liquid during storage was investigated. ‒THC‒OAc liquids in cartridges were stored at 80℃ or 30℃ for 28 days. The relative concentrations of cannabinoids in the liquids were measured by gas chromatography with flame ionization detection. ‒THC‒OAc decreased during storage at either temperature. ‒THC, a minor impurity, also decreased. ‒THC‒OAc liquid in a cartridge was stored at 80℃ for 57 days. The concentration of ‒THC‒OAc and ‒THC, a minor impurity, did not change during that time. Cannabinol acetate increased in all liquids, and unknown products were also detected by gas chromatography/mass spectrometry (GC/MS). The ‒THC‒OAc and ‒THC‒OAc liquids were subjected to GC/MS to detect representative diluents, glycerol, propylene glycol, and polyethylene glycol 400, but none of these were detected.

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To obtain fundamental data for research on the transmutation of nuclear waste, the thermal neutron cross section and the resonance integral of the reaction

99Tc

(n, γ)¹⁰⁰Tc have been measured using an activation method.
Four ammonium pertechnetate targets containing 371375 kBq of

99Tc

were irradiated for 2min with reactor neutrons. Activation detectors of Co/Al and Au/Al alloy wires were irradiated for 10min to monitor the neutron flux and the fraction of the epithermal part (Westcott' sepithermal index). The Tc samples and flux monitors were irradiated with and without a Cd capsule. The γ-ray spectra from the irradiated samples were measured using a high purity Ge detector.
The thermal neutron cross section (2, 200m/s neutron cross section) and the resonance integral of the

99Tc

(n, γ)¹⁰⁰Tc reaction were found to be

22

.

9

±1.3

b

and 398±38

b

, respectively.
The thermal neutron cross section obtained agrees with the previously reported values (20±2

b

by Lucas, 24.

8

by Pattenden, 24±4 by Ovechkin) within the limits of error. On the other hand, the resonance integral is twice the value reported by Lucas (186±16

b

).

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The β-ray spectra of Ni⁶³, Zn⁶³, Zn⁶⁵, Zn⁶⁹ (57 m), Zn⁶⁹ (13. h), Ga⁶⁸, Ga⁷⁰, Ga⁷², Ge⁷¹, Ge⁷⁵ and Ge⁷⁷ have been investigated, by measuring the curvature of the tracks in a cloud chamber situated in a known magnetic field. The upper limit derived from K-U plots are summarised as follows.
Ni⁶³ 2.5h - 1.65 × 10⁶ eV .67 × 10⁶ eV
Zn⁶³ 35m + 1.92 × 10⁶ eV
Zn⁶⁵ 210d + .37 × 10⁶ eV .19 × 10⁶ eV
Zn⁶⁹ 57m - . × 10⁶ eV
Zn⁶⁹ 13.h - . × 10⁶ eV
Ga⁶⁸ 66m + 1.37 × 10⁶ eV
Ga⁷⁰ 20m - 1.68 × 10⁶ eV
Ga⁷² 14h - 1.71 × 10⁶ eV
Ge⁷¹ 26h + 1.15 × 10⁶ eV
Ge⁷⁵ 81m - 1.10 × 10⁶ eV
Ge⁷⁷ h - 1.92 × 10⁶ eV

From the consideration of energy balance, the minimum neutron energies to produce neutron loss processes-, for Cu⁶², Zn⁶³ and Ga⁶⁸ are estimated roughly to be 12.7, . and . MeV respectively, which are in good agreement with the values reported by one of the authors except the last case.

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