Mycobacteriocin produced by human type tubercle bacilli could be demonstrated easily on eggmedium containing 0.05% tween 80 by means of stab culture or streak plate methods, and elevenmycobacteriocin-types have been recognized on the basis of the inhibition pattern by using
9
indicator strains of rapidly growing mycobacteria.
At present, types 1 (
4
.
9
%), 2 (13%),
4
(38.
5
%),
9
(2%) and 11 (14.8%) showed a stablemycobacteriocin pattern, but types 3 (
7
.8%) and
7
(
6
.
7
%) were apt to show type replacement totypes 2 and
4
, respectively, and strains belonging to types
5
,
6
and 8 were few, less than 1.
5
%to the total. In order to perform a reproducible mycobacteriocin typing, our previous typingscheme was revised to be consisted of five groups (A-
E
). Among 438 strains, mycobacteriocingroup C containing types
4
and
7
was 46.1% of strains tested, group
B
containing types 2, 3 and
6
was
22
.
6
%, group
E
containing type 11 was 15.1%, group A containing types 1 and 8 was
5
.
5
%, group
D
containing types
9
and 10 was
4
.1%, and untypable strains were no more than
6
.
6
% among all tested strains.
Human type tubercle bacilli classified as type
4
, which consisted of more than 45% of strainstested, could be divided into three sub-types from sensitivity to M. gordonae 1324 and M. terrae 1450.
Mycobacteriocin producing strains belonging to type
D
:
9
showed an extremly wide antibacterialactivity to strains of more than
90
% of rapidly growing mycobacteria (42 strains), to about 40% of slow growing mycobacteria (29 strains), and even to 10 strains of
Staphylococcus aureus.
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