抄録
We developed a microphotometric system for multispectral image analysis. This equipment is employed when several colored components are deposited in the specimen and their spectra are overlapped to each other extensively.
The spectral images of the specimen were obtained by the use of ten pieces of interference filter, CCD camera and frame grabber (640×480 pixel units of 8 bit density). From both the spectral profiles of individual colored components and the multispectral images of specimen, the spatial pattern of components was reconstructed quantitatively with our proposed system.
To evaluate the applicability of this system to the practical histochemistry, we examined the images of histochemically stained specimens; Rat liver section stained with hematoxylin-eosin and a ciliated protozoa, Paramecium caudatum stained in combination of nitroblue tetrazolium (for determination of succinate dehydrogenase activity) and naphthol yellow S (for tissue protein).
Instrumentation, principle and application of our proposed system are described in this paper.
