巻号一覧
24 巻 (1991)
- 6 号 p. 545-
- 5 号 p. 449-
- 4 号 p. 377-
- 3 号 p. 269-
- 2 号 p. 135-
- 1 号 p. 1-
24 巻, 4 号
選択された号の論文の9件中1～9を表示しています
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SHIGEAKI MORI, HATSUMI FUJII, TAKASHI YASUE, MASARU OHASHI, HARUO OHTA ...1991 年 24 巻 4 号 p. 377-387
発行日: 1991年
公開日: 2009/10/28
ジャーナル フリーThe immunologic characteristics of vascular endothelial cells (EC) in cutaneous T-cell malignancies were studied immunocytochemically at light and electron microscopic levels. In cutaneous lesion of adult T-cell lymphoma, a large number of small vessels with flat EC was distributed, whereas another types of cutaneous T-cell malignancies were rich in high endothelial venules (HEV). They were HLA-DR+, OKM5+, IL-1+ and von Willebrand factor antigen (FVIII RAg/vWF)+. The upper dermis of normal and inflammatory skin disease was rich in HEV, which were HLA-DR+, OKM5-, IL-1+ and FVIII RAg/vWF+. Lymphoma cells, regardless of the histologic type, were CD3+, HLA-DR+ and predominantly CD4+. About one third of CD3+ cells displayed IL-2 receptor. We discussed the possibility that the EC in cutaneous T-cell lymphoma may allow the proliferation of T lymphoma cells, which also have been known to induce angiogenesis.抄録全体を表示PDF形式でダウンロード (16193K) -
MASAMI OGUNI1991 年 24 巻 4 号 p. 389-394
発行日: 1991年
公開日: 2009/10/28
ジャーナル フリーThe appearance of two molecular markers of muscle differentiation, brain (BGP) and muscle-brain (MB-GP) type of glycogen phosphorylase, was studied immunohistochemically in the extraocular muscles of human embryos (Carnegie stages 13 to 23). During stages 13-18, there was no immunoreactivity to BGP and MB-GP antibodies around the optic vesicle. At stage 20, myogenic cells around the optic vesicle became immunoreactive to both BGP and MB-GP antibodies, however, the number of MB-GP immunoreactive cells was larger than that of BGP immunoreactive cells. At stage 21, MB-GP immunoreactive cells increased in number, but BGP immunoreactive cells decreased. At stage 23, BGP immunoreactivity disappeared from the extraocular muscles. These findings suggest that BGP appears transiently in a restricted population of extraocular muscles in the embryonic period and the isoenzyme pattern becomes adult type (MGP) in the late embryonic period.抄録全体を表示PDF形式でダウンロード (3896K) -
KENSAUKE CHIKAMORI, AKIRA YAMAMOTO, TSUTOMU ARAKI1991 年 24 巻 4 号 p. 395-403
発行日: 1991年
公開日: 2009/10/28
ジャーナル フリーWe developed a microphotometric system for multispectral image analysis. This equipment is employed when several colored components are deposited in the specimen and their spectra are overlapped to each other extensively.
The spectral images of the specimen were obtained by the use of ten pieces of interference filter, CCD camera and frame grabber (640×480 pixel units of 8 bit density). From both the spectral profiles of individual colored components and the multispectral images of specimen, the spatial pattern of components was reconstructed quantitatively with our proposed system.
To evaluate the applicability of this system to the practical histochemistry, we examined the images of histochemically stained specimens; Rat liver section stained with hematoxylin-eosin and a ciliated protozoa, Paramecium caudatum stained in combination of nitroblue tetrazolium (for determination of succinate dehydrogenase activity) and naphthol yellow S (for tissue protein).
Instrumentation, principle and application of our proposed system are described in this paper.抄録全体を表示PDF形式でダウンロード (6498K) -
MINGQIU GUO, RONGCHENG LUO, XUEMEI DING, KANGXIAN LUO, LIAN ZHAN, YING ...1991 年 24 巻 4 号 p. 405-409
発行日: 1991年
公開日: 2009/10/28
ジャーナル フリーImmunohistochemical expression of interleukin 2 receptor (IL2R) is examined by an APAAP method in the peripheral blood mononuclear cells (PBMC) in asymptomatic HBsAg carriers (AsC) and in normal individuals. It is found that the percentage of IL2R-positive cells is significantly lower in PBMC induced by HBsAg or ConA in AsC, but not so by PHA, than that in normal individuals (P<0.01). Moreover, it is found that IL2R-positive cell is surrounded by many IL2R-negative mononuclear cells following induction by HBsAg. This phenomenon is temporarily termed IL2R-positive cell “rosette”. Typical rosette formation of IL2R-positive cells is not observed in induction by ConA and PHA. Besides, when the percentage of IL2R-positive cell rosette is counted, the results show that the prevalence of rosette formation is much higher in normal individuals than that in AsC (P<0.01). This new phenomenon of immune response to HBsAg may be of important significance in immunoregulation. The results suggest that the abnormal IL-2R expression and immunoregulation of IL2R-positive cell may be an important mechanism of poor immune response to hepatitis B virus (HBV) in AsC.抄録全体を表示PDF形式でダウンロード (2702K) -
TOSHIO YAMAMOTO, HIROSHI NAGAI, CAROL V. GAY1991 年 24 巻 4 号 p. 411-419
発行日: 1991年
公開日: 2009/10/28
ジャーナル フリーCytochrome oxidase activity in rat metaphyseal bone cells was determined by enzyme cytochemistry. Factors such as fixation, incubation time and permeability of diaminobenzidine and cytochrome c, which might affect cytochemical reactivity of the enzyme, were evaluated. Three levels of cytochrome oxidase reactivity were observed in the mitochondria of each type of bone cell. These were 1) staining of the entire membrane system of mitochondria (type I), 2) partial staining in which only cristae were stained (type II) and 3) no staining (type III). In most osteoclasts, type I mitochondria predominated, and types II and III were few in number. Osteoblasts possessed mixed populations of mitochondria consisting mainly of types I and II, but some osteoblasts appeared to be in a functional transition and contained mitochondria weak in activity. Osteocytes had largely type III and few type II mitochondria. The results indicate that heterogeneity of cytochrome oxidase activity exists in bone cell mitochondria and that osteoclasts have the most active aerobic metabolism among bone cells, whereas osteoblasts favor oxidative metabolism and osteocytes are anaerobic.抄録全体を表示PDF形式でダウンロード (12261K) -
NOBUO MORIYAMA, KAZUKI KAWABE, KOZO HAMADA, KIYOSHI KIMURA, ISAO MURAH ...1991 年 24 巻 4 号 p. 421-426
発行日: 1991年
公開日: 2009/10/28
ジャーナル フリーThe α1-adrenoceptor is considered to regulate micturition in patients with benign prostatic hypertrophy. This study demonstrated the specific binding sites of [3H]-bunazosin hydrochloride (4-amino-2-2-[4-butanoyl-hexahydro-1H-1, 4-diazepin-1-yl]-6, 7-dimethoxy quinazoline hydrochloride) in the large sections of the prostate. Eleven surgical specimens of en bloc retropubic prostatectomy were evaluated by in vitro receptor autoradiography. Autoradiograms were assessed using a computerized image analysis system to locate the specific binding sites. Suitable specimens were coated with emulsion for microautoradiography. Specific binding sites were recognized mainly in the perinodular regions of the prostate as well as in some nodules and in the urethral region. Silver grains were recognized in the fibromuscular stroma of the perinodular and subepithelial regions of the prostatic ducts and acini. Some epithelial cells and the basement membrane of the ducts, the acini, and the prostatic urethra also revealed the accumulation of silver grains. Whether this latter finding indicated specific binding to α1-adrenoceptors or not is a matter for further investigation. This study indicated that [3H]-bunazosin was useful for the qualitative demonstration of specific α1-adrenoceptor binding sites in the prostate by in vitro receptor autoradiography.抄録全体を表示PDF形式でダウンロード (10024K) -
EFFECT OF ANTI-ANDROGENSMASANORI MURAKOSHI, RIE INADA, MASASHI TAGAWA, MASAHARU SHOUJI, MINORU ...1991 年 24 巻 4 号 p. 427-434
発行日: 1991年
公開日: 2009/10/28
ジャーナル フリーIn order to confirm the relationship between glutathione-peroxidase (GSH-PO) and testosterone action in rat ventral prostate, we have studied the immunocytochemical localization of GSH-PO in glandular epithelial cells of rat ventral prostate after treatment of TZP-4238 or chlormadinone acetate (CMA) as antiandrogens. In the control rat ventral prostate, GSH-PO was predominantly observed in the glandular epithelial cells and intracellular localization of GSH-PO was mainly observed in the cytoplasmic matrix near the rough endoplasmic reticulum and it was occasionally noted as a small granular structure at the supranuclear region. In TZP-4238-or CMA-administered rats, the glandular epithelial cells of the ventral prostate were markedly atrophic. The intensity of GSH-PO staining in the glandular epithelial cells was markedly decreased. Immunoelectron microscopically, GSH-PO-positive granules were hardly seen in the atrophic glandular epithelial cells. These findings strongly suggest that loss of GSH-PO staining in the glandular epithelial cells of the rat ventral prostate treated with TZP-4238 or CMA as anti-androgens is thought to be caused by inhibition of testosterone action, and that its staining pattern is a useful for the effect of antiandrogens on prostate.抄録全体を表示PDF形式でダウンロード (9892K) -
WEN HU, YINGJIE PIAO, YIMO ZHOU, KAZUO OGAWA1991 年 24 巻 4 号 p. 435-439
発行日: 1991年
公開日: 2009/10/28
ジャーナル フリーWe have investigated the lysosomophagy in mouse peritoneal macrophages after the endocytosis of colloidal gold-labeled Concanavalin A (ConA-Au). The mice were injected intraperitoneally with ConA-Au. At different intervals of time (5-120min), peritoneal macrophages were isolated and observed under an electron microscope. Besides the active endocytosis of ConA, a large number of lysosomes appeared and were digested by other lysosomes after 20-30min incubation (lysosomophagy, a kind of lysosomal wrapping mechanism). We concluded that an active heterophagy could induce excessive multiplication of lysosomes. In order to maintain the balance of lysosomal system, these excessive lysosomes were finally degraded by other lysosomes.抄録全体を表示PDF形式でダウンロード (2526K) -
TOSHIKO NAGASHIMA, HITOSHI TANABE, YASUNORI FUJIOKA, KAZUO NAGASHIMA, ...1991 年 24 巻 4 号 p. 441-446
発行日: 1991年
公開日: 2009/10/28
ジャーナル フリーLocalization of protein kinase C (PKC) isozymes in the human nervous system, focused upon the cerebellum, hippocampus, and spinal cord was studied with monoclonal antibodies (MC-1a, -2a, -3a) against isozymes types I, II and III of PKC, respectively. Tissues were sliced by cryostat and fixed with paraformaldehyde, incubated with these antibodies, and processed for avidin-biotin peroxidase complex staining. Type I was densely localized in the cytoplasm of Purkinje cells, with their dendrites and axon terminals around the dentate nucleus of the cerebellum. Neurons of the hippocampus, especially in CA-1 to 3, and anterior horn cells of the spinal cord were also stained positively with type 1. Type II was localized randomly in some neurons of the hippocampus (CA-2 and 3), and in the substantia gelatinosa in the posterior horn of the spinal cord. Type III was diffusely present in glial tissues of the perivascular region of the brain and in the subpial layer of the spinal cord. PKC isozyme immunohistochemistry can be applied to human autopsy materials. The distinct localizations of PKC isozymes in human nervous tissues may suggest different roles in signal transduction for regulation of different cellular responses, and it may cause either normal or pathologic processes.抄録全体を表示PDF形式でダウンロード (7774K)
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