抄録
Little is known about the spectral characteristics of leucocytes stained by routine methods and their values in blood cytodiagnosis. In this work a new method was developed for measuring color differences per cell between different cells, and the applicability of this method in characterization of leucocyte types was examined. In this new method the relative color spectrum was measured over the range of 370 nm to 700 nm as the ratio of the absorption at each wavelength to the maximum absorption (A/Amax, %). The relative color spectrum was obtained from the absorption curve by a wavelength-scanning method using a double-beam microspectrophotometer with a projection beam area larger than the cell size. The error due to staining was least when blood cells are stained by a modification of the method of Wright. Several types of human and animal leucocytes were distinguished on the basis of their relative color spectra, and these spectral differences seemed suitable for blood cytodiagnostic purposes, because a fixed spectral pattern was obtained for most leucocyte types, even including lobated-nuclear neutrophils. However, it has not yet been possible to distinguish eosinophils from pseudoeosinophils in fowl blood or monocytes from some lymphocytes.
