抄録
The glycoside composition and sequence of an extracellular polysaccharide flocculant of
Klebsiella pneumoniae H12 was analyzed. GC and HPLC analysis of the acid-hydrolysate identified its constituent monosaccharides as
D-Glc,
D-Man,
D-Gal, and
D-GlcA in an approximate molar ratio of 3.9:1.0:2.3:3.6. To analyze the glycoside sequence, the polysaccharide was partially hydrolyzed by acid and enzyme treatment. GC, HPLC, TLC, MALDI-TOF/MS, and 1H- and 13C- NMR spectroscopy characterized the obtained oligosaccharides.
The results clarified the partial structure of H12 polysaccharide as a linear polymer of a unit of pentasaccharide with a side chain of one
D-GlcA to
D-Glc moiety (see below). Although the existence of other sequences or other constituent glycosides could not be fully excluded, H12 polysaccharide must be a novel types as such a complicated unit for a polymer has not so far been reported. The partial structure of a H12 polysaccharide flocculant is also discussed in this report.
| →4)- | α-D-Glcp-(1→ | 2)-α-D-Manp-(1→3)-4,6-Pyr-β-D- |
| | 3 | Galp-(1→4)-β-D-Galp-(1→ |
| | ↓
1 | |
| | β-D-GlcpA
| |
