Cloning of Structural Gene of Deinococcus radiodurans UV-Endonuclease β

Shigeru KITAYAMA; Issay NARUMI; Tomoo FUNAYAMA; Hiroshi WATANABE

doi:10.1271/bbb.67.613

Biochemistry & Molecular Biology Notes

Cloning of Structural Gene of Deinococcus radiodurans UV-Endonuclease β

Shigeru KITAYAMA, Issay NARUMI, Tomoo FUNAYAMA, Hiroshi WATANABE

著者情報

キーワード: Deinococcus radiodurans, DNA repair gene, endonuclease, flap endonuclease 1, UV-endonuclease β

ジャーナルフリー

2003 年 67 巻 3 号 p. 613-616

DOI https://doi.org/10.1271/bbb.67.613

詳細

抄録

To characterize its enzymic property we cloned and sequenced the gene of Deinococcus radiodurans encoding UV-endonuclease β, an alternative enzyme to UvrABC repairing damaged DNA. Amino acid substitutions were found in UV-sensitive mutants. The putative amino acid sequence had some similarity with those of eukaryotic UV-endonucleases and with a sequence found in a protein data base of Bacillus subtilis.

責任著者(Corresponding author)

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