Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
Reverse Transcriptase-Like Sequences Related to Retrotransposon in a Red Alga, Porphyra yezoensis
Wenbo ZHANGMika SAKAIXiaofei LINKatsuaki TAKECHIHiroyoshi TAKANOSusumu TAKIO
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2006 年 70 巻 8 号 p. 1999-2003

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Four DNA fragments encoding a reverse transcriptase (RT)-like gene related to that of long terminal repeat (LTR) retrotransposons were isolated from the red alga Porphyra yezoensis by genomic PCR. Southern blot analysis suggested that one clone exists as a single copy per genome. Its full-length cDNA (PyRE2A) contained RT/RNase H-like sequences, which are most closely related to those of the Volvox LTR retrotransposon, although two stop codons were present within the RT region. We did not find any sequence related to LTR retrotransposons other than RT/RNase H in RyRE2A. These results indicate that PyRE2A is a single RT/RNase H-like gene and a defective progenitor of LTR retrotransposons.
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© 2006 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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