Molecular Cloning and Sequence Analysis of an Extracellular Protease from Four Bacillus subtilis Strains

Xujun HAN; Yuh SHIWA; Masanori ITOH; Tohru SUZUKI; Hirofumi YOSHIKAWA; Toshiyuki NAKAGAWA; Hiroko NAGANO

doi:10.1271/bbb.120920

Biochemistry & Molecular Biology Notes

Molecular Cloning and Sequence Analysis of an Extracellular Protease from Four Bacillus subtilis Strains

Xujun HAN, Yuh SHIWA, Masanori ITOH, Tohru SUZUKI, Hirofumi YOSHIKAWA, Toshiyuki NAKAGAWA, Hiroko NAGANO

著者情報

キーワード: Bacillus subtilis, alkaline protease, clone, activity

ジャーナルフリー

2013 年 77 巻 4 号 p. 870-873

DOI https://doi.org/10.1271/bbb.120920

View "Advance Publication" version (April 7, 2013).

詳細

抄録

Four Bacillus subtilis strains were isolated from traditional fermented foods, and the sequences of their extracellular alkaline proteases (AprE) were analyzed and cloned. The recombinant enzymes synthesized by means of Escherichia coli exhibited high proteolytic activity. AprE CN2 showed hydrolytic activity 4-fold higher than that of AprE 168. This activity was also seen in the presence of relatively high NaCl concentrations.

責任著者(Corresponding author)

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