Molecular Cloning and Sequence Analysis of an Extracellular Protease from Four Bacillus subtilis Strains

Xujun HAN; Yuh SHIWA; Masanori ITOH; Tohru SUZUKI; Hirofumi YOSHIKAWA; Toshiyuki NAKAGAWA; Hiroko NAGANO

doi:10.1271/bbb.120920

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Molecular Cloning and Sequence Analysis of an Extracellular Protease from Four Bacillus subtilis Strains

Xujun HAN, Yuh SHIWA, Masanori ITOH, Tohru SUZUKI, Hirofumi YOSHIKAWA, Toshiyuki NAKAGAWA, Hiroko NAGANO

著者情報

キーワード: Bacillus subtilis, alkaline protease, clone, activity

ジャーナルフリー早期公開

論文ID: 120920

DOI https://doi.org/10.1271/bbb.120920

この記事には本公開記事があります。

The final version of this article is now available: Vol. 77 (2013) , No. 4 p.870

詳細

抄録

Four Bacillus subtilis strains were isolated from traditional fermented foods, and the sequences of their extracellular alkaline proteases (AprE) were analyzed and cloned. The recombinant enzymes synthesized by means of Escherichia coli exhibited high proteolytic activity. AprE CN2 showed hydrolytic activity 4-fold higher than that of AprE 168. This activity was also seen in the presence of relatively high NaCl concentrations.

責任著者(Corresponding author)

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