The Contribution of Peripheral Stem-Loops to the Catalytic Activity of Archaeal RNase P RNA from Pyrococcus horikoshii OT3

Tadashi HARA; Atsushi TERADA; Hiroki YAMAGUCHI; Takashi NAKASHIMA; Yoshimitsu KAKUTA; Makoto KIMURA

doi:10.1271/bbb.110110

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The Contribution of Peripheral Stem-Loops to the Catalytic Activity of Archaeal RNase P RNA from Pyrococcus horikoshii OT3

Tadashi HARA, Atsushi TERADA, Hiroki YAMAGUCHI, Takashi NAKASHIMA, Yoshimitsu KAKUTA, Makoto KIMURA

著者情報

キーワード: archaea, Pyrococcus horikoshii, protein-RNA interaction, ribonucleoprotein, RNase P RNA

ジャーナルフリー早期公開

論文ID: 110110

DOI https://doi.org/10.1271/bbb.110110

この記事には本公開記事があります。

The final version of this article is now available: Vol. 75 (2011) , No. 4 p.816

詳細

抄録

We investigated the contribution of peripheral stem-loops to the catalytic activity of an archaeal RNase P RNA, PhopRNA, from Pyrococcus horikoshii OT3. PhopRNA mutants, in which the stem-loops were individually deleted, were prepared and characterized with respect to precursor tRNA (pre-tRNA) cleavage activity in the presence of five RNase P proteins. All the mutants retained the activity to some extent, indicating that they are moderately implicated in catalysis. Further characterization suggested that the stem-loops serve largely as binding sites for the proteins, and that their interactions are predominantly involved in stabilization of the active conformation of PhopRNA.

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