Membrane Topology and Functional Analysis of Methylobacillus sp. 12S Genes epsF and epsG, Encoding Polysaccharide Chain-Length Determining Proteins

Yuko AYABE-CHUJO; Yusuke USAMI; Takako YOSHIDA; Toshio OMORI; Hideaki NOJIRI

doi:10.1271/bbb.110831

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Membrane Topology and Functional Analysis of Methylobacillus sp. 12S Genes epsF and epsG, Encoding Polysaccharide Chain-Length Determining Proteins

Yuko AYABE-CHUJO, Yusuke USAMI, Takako YOSHIDA, Toshio OMORI, Hideaki NOJIRI

著者情報

キーワード: extracellular polysaccharide, chain-length, membrane topology, Methylobacillus, phosphorylation

ジャーナルフリー早期公開

論文ID: 110831

DOI https://doi.org/10.1271/bbb.110831

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2012) , No. 3 p.608

詳細

抄録

The EpsF and EpsG of the methanol-assimilating bacterium Methylobacillus sp. 12S are involved in the synthesis of a high molecular weight exopolysaccharide, methanolan. These proteins share homology with chain-length determiners in other polysaccharide-producing bacteria. The N- and C-termini of EpsF were found to locate to the cytoplasm, and EpsF was predicted to have two transmembrane regions. EpsG showed both ATPase and autophosphorylation activities.

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