Expression and Characterization of a Thermostable Acetylxylan Esterase from Caldanaerobacter subterraneus subsp. tengcongensis Involved in the Degradation of Insoluble Cellulose Acetate

Kunihiko MORIYOSHI; Daisuke KOMA; Hayato YAMANAKA; Kiyofumi SAKAI; Takashi OHMOTO

doi:10.1271/bbb.130568

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Expression and Characterization of a Thermostable Acetylxylan Esterase from Caldanaerobacter subterraneus subsp. tengcongensis Involved in the Degradation of Insoluble Cellulose Acetate

Kunihiko MORIYOSHI, Daisuke KOMA, Hayato YAMANAKA, Kiyofumi SAKAI, Takashi OHMOTO

著者情報

キーワード: cellulose acetate, biodegradation, acetylxylan esterase, thermostable, Caldanaerobacter subterraneus

ジャーナルフリー早期公開

論文ID: 130568

DOI https://doi.org/10.1271/bbb.130568

この記事には本公開記事があります。

The final version of this article is now available: Vol. 77 (2013), No. 12 pp. 2495-2498

詳細

抄録

A thermostable acetylxylan esterase gene, TTE0866, which catalyzes the deacetylation of cellulose acetate, was cloned from the genome of Caldanaerobacter subterraneus subsp. tengcongensis. The pH and temperature optima were 8.0 and 60 °C. The esterase was inhibited by phenylmethylsulfonyl fluoride. A mixture of the esterase and cellulolytic enzymes efficiently degraded insoluble cellulose acetate with a higher degree of substitution.

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