Identification of Proteins Interacting with Selenocysteine Lyase

Ryuta TOBE; Hisaaki MIHARA; Tatsuo KURIHARA; Nobuyoshi ESAKI

doi:10.1271/bbb.90065

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引用する場合も本公開記事を引用してください。

Identification of Proteins Interacting with Selenocysteine Lyase

Ryuta TOBE, Hisaaki MIHARA, Tatsuo KURIHARA, Nobuyoshi ESAKI

著者情報

キーワード: selenocysteine lyase, protein-protein interaction, yeast two-hybrid system, major urinary protein, selenophosphate synthetase

ジャーナルフリー早期公開

論文ID: 90065

DOI https://doi.org/10.1271/bbb.90065

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2009) , No. 5 p.1230

詳細

抄録

Yeast two-hybrid screening of mouse cDNA libraries was performed to identify proteins interacting with selenocysteine lyase (SCL), which decomposes L-selenocysteine. Several proteins related to spermatogenesis, protein synthesis, and cell viability/apoptosis were identified as potential interactors. Major urinary proteins 1 and 2 interacted with SCL and inhibited its activity. Coimmunoprecipitation revealed interactions between SCL and each of two selenophosphate synthetase isozymes.

責任著者(Corresponding author)

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