Abstract
1. It has been confirmed that Ogur and Rosen's method can be applied to the determination of nucleic acids of dried yeasts, moulds and bacteria.
2. It has been found that their method gives erroneous results when applied directly to fresh or durable yeasts.
3. Correct results have been obtained when fresh or durable, yeasts were subjected to freezing overnight with dry ice and ether.
4. In order to effect the complete extraction of nucleic acid, such pre- liminary treatments as autolysis, homogenizing with Pyrex glass pow- der, and alternate repetitions of dry ice -freezing and melting have been tried with no promising results.
5. Based on the experimental results, a most reliable procedure for the determination of nucleic acids of microorganisms has been described.
6. Analytical data of RNA and DNA in various microorganisms obtained by the present method has been pre- sented.
