The factors influencing the formation of adaptive enzymes,
m-hydroxybenzoic acid oxidase and gentisase, in the secondary adaptation procedure by the non-proliferating cells of
Pseudomonas ovalis var. S-5 were examined and the following results obtained.
1) The addition of
m-hydroxyben-zoic acid to the culture media, at the time of initiation of incubation. and at three hours before the harvest of the cells, decreases the time required foradaptive enzyme formation in the se-condary adaptation, i.e., in the former case 1 to 2 hours and in the latter case o to 1 hour is enough for the completion of the induction of both enzymes.
2) The optimum length of time required for the completion of the induction of both enzymes under the standard conditions of the secondary adaptation procedure was approximate-ly three hours.
3) The optimum concentration of substrate,
m-hydroxybenzoic acid, in the secondary adaptation procedure was M/100 for
m-hydroxybenzoic acid oxidase and M/200 for gentisase.
4) No effect of the addition of glucose as the energy source upon the induction of gentisase and
m-hydroxy-benzoic acid oxidase was recognized with the cells adapted for three hours in the shaking flask or with the cells grown in bouillon glucose medium. However, the effect was recognized with the cells adapted for one hour in shaking flasks or with the cells adapted in Warburg vessels.
The authors wish to express their sincerest thanks to Prof. K. Sakaguchi, for his guidance and encouragement throughout these research, and also wish to thanks Assist Prof. H. Taka hashi for his useful discussion.
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