Enzymatic Resolution of Racemic Amino Acids

Part IV. Another Purification Method of Mold Acylase and Effect of Metal Ions, Cyanide and EDTA on the Enzyme Activity

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The mold acylase has been purified by precipitation of the enzyme-rivanol complex and elution from the complex, together with fractionation with ammonium sulfate and acetone.
The mold acylase appears to be specifically activated by Co⁺⁺ ion and inactivated by Mn⁺⁺ and Hg⁺⁺ ions as well as by EDTA.
The enzyme activity inhibited by EDTA was restored to its original level with the addition of the cobaltous ion.
This research was aided by a grant from the Ajinomoto Company.