抄録
1) In the case of yeast, it has been demon-strated that acid-soluble compounds are not sufficiently eliminated when extracted twice with 0.2 N PCA according to Ogur and Rosen's method.
2) Even if the extraction with 0.2 N PCA is repeated further, acid-soluble compounds are not extracted completely; on the contrary, RNA begins to dissolve into the acid-soluble fraction.
3) It has been shown that these defects can be eliminated only when acid-soluble com-pounds are extracted with water instead of with 0.2 N PCA.
4) Based on the facts described above, the previous report on the determination of nucleic acids in fresh or durable yeast has been further modified, from the viewpoint of elution behavior of acid-soluble compounds.
