抄録
A sensitive spectrophotometric method for qualitative and quantitative determination of D-arabinose and L-fucose in the 0.02_??_0.1 μmole range is described. Enzyme used for this assay is n-arabinose (L-fucose): NAD 1-oxidoreductase (EC. 1. 1. 1. 116) prepared from the D-arabinose-grown cells of Pseudomonas sp. The enzyme accepts either D-arabinose or L-fucose as substrate and is required NAD or NADP as coenzyme. D-Arabinose and L-fucose can be determined as the rate of NAD reduction at 340 nm at 30°C with reaction velocity method and end point method. This assay method is highly specific and 1, 000-fold excess of following sugars and sugar alcohols do not interfere the determination of microquantity (10-4M) of D-arabinose and L-fucose: L-arabinose, D-xylose, D-ribose, D-Iyxose, D-fucose, L-rhamnose, D-fructose, sucrose, xylitol, L-arabitol, D-arabitol, ribitol, sorbitol and D-mannitol. Moreover, L-fucose can be recovered in the presence of 4, 000-fold excess of D-galactose and D-mannose. D-Arabinose and L-fucose can be identified and determined separately by this method.
