総説
生きた細胞内に人工のハイドロゲル凝縮体をつくる合成生物学技術iPOLYMER
キーワード:
synthetic biology,
biomolecular condensates,
phase transition/separation,
hydrogels,
stress granules,
iPOLYMER/iPOLYMER-LI
2021 年 61 巻 3 号 p. 166-170
詳細
2021 年 61 巻 3 号 p. 166-170
In a previous report, the author developed a synthetic biology molecular tool, iPOLYMER, which produces hydrogel condensates consisting of two polypeptide chains in living cells, in a stimulus-dependent manner. Functionalizing the iPOLYMER polypeptides with an RNA-binding domain successfully reconstituted a typical example of biomolecular condensates, stress granules, in living cells under a stress-free condition. In the current review, achievements made possible by iPOLYMER are summarized, with reference to a brief history of biomolecular condensate research. Future prospects, as well as technical challenges, of synthetic biological approaches to the novel class of intracellular structures are also described.
