Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Simultaneous Determination of Glycyrrhizin, Glycyrrhetic Acid and Glycyrrhetic Acid Mono-Glucuronide in Shakuyaku-kanzo-to Incubated with Rat Feces by Semi-micro High-Performance Liquid Chromatogaphy
Nobuyuki OKAMURATomonori MAKIHidetoshi MIYAUCHIMiyuki SHIMOEShizuko YOKONOHironori YOSHITOMIAkira YAGI
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2001 Volume 24 Issue 10 Pages 1161-1164


A method for semi-micro high-performance liquid chromatography (HPLC) has been established for the simultaneous determination of glycyrrhizin (GL), glycyrrhetic acid (GA) and glycyrrhetic acid mono-glucuronide (GAMG) in incubation mixtures of rat feces with Shakuyaku-kanzo-to decoction (combination of licorice root and peony root). The analysis could be accomplished within 20 min with a TSKgel ODS-80TsQA (150×2.0 mm i.d.) column by linear gradient elution using a mobile phase containing aqueous phosphoric acid and acetonitrile at a flow rate of 0.2 ml·min-1, a thermostatic oven at 25°C, and detection at 254 nm. The detection limits of these compounds were 0.1—0.85 pmol per injection (5 μl). The concentrations of GL and its metabolites in the incubation mixture after continuous consumption of Shakuyaku-kanzo-to were significantly different compared with those of untreated control. GL-hydrolysis of rat feces was enhanced by pre-consumption of Shakuyaku-kanzo-to.

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© 2001 The Pharmaceutical Society of Japan
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