Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Double Staining of Ginsenosides by Western Blotting Using Anti-ginsenoside Rb1 and Rg1 Monoclonal Antibodies
Noriko FUKUDAHiroyuki TANAKAYukihiro SHOYAMA
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2001 Volume 24 Issue 10 Pages 1157-1160

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Abstract

Ginsenosides separated by silica gel TLC blotted to a polyvinylidene difluoride (PVDF) membrane was treated with a NaIO4 solution followed by bovine serum albumin (BSA), resulting in a ginsenoside-BSA conjugate on a PVDF membrane. The blotted bands were stained with anti-ginsenoside Rg1 and Rb1 monoclonal antibodies (MAbs). The newly established double staining with the Western blotting methods was applied for the determination of ginsenosides possessing protopanaxadiol or protopanaxatriol and the number of sugar depending on the stained color and their Rf values in the Panax plants.

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© 2001 The Pharmaceutical Society of Japan
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