Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158

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Mechanisms of Yangjing capsule in Leydig cell apoptosis and testosterone synthesis via promoting StAR expression
Dalin SunWeihang DongBaofang Jin Guanghui ChenBin CaiWeimin DengYugui CuiYihan Jin
著者情報
キーワード: YC, Leydig cell, apoptosis, testosterone, StAR
ジャーナル フリー 早期公開

論文ID: b18-00205

この記事には本公開記事があります。
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The present study aims to investigate the roles of StAR in Yangjing Capsule (YC) induced anti-apoptotic effects on Leydig cells and the related mechanism. Leydig tumor cells (MLTC-1) were cultured and treated with YC, and immunofluorescence assay was performed to examine the expression of StAR; furthermore, luciferase reporter assay was conducted to evaluate the impact of YC on StAR promoter; next, MLTC-1 cells were treated with StAR siRNA, and flow cytometry was carried out to examine the effect of StAR siRNA on the apoptosis of the cells; furthermore, qRT-PCR and western blot methods was used to determine the expression of StAR and apoptosis related molecules Bcl-2, Bax and Caspase-3 on both mRNA and protein levels in different groups; finally the secretion of testosterone in different groups was examined by radioimmunoassay. We observed that the YC can increase the expression of StAR in a dose-dependent manner, and YC can activate the promoter of StAR;moreover, transfection of StAR siRNA can block YC induced anti-apoptotic effects and increased production of testosterone. In conclusion, our results suggested that YC might suppress the apoptosis of MLTC-1 cells and enhance the production of testosterone through regulating the expression of StAR.

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© 2018 The Pharmaceutical Society of Japan
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