Abstract
Two subclones (K562-L and -H) were previously isolated from K562 human leukemic cells according to hemoglobin production : K562-L was expressed in less than 5% and K562-H in more than 90% of dianisidine positive cells. 12-O-Tetradecanoylphorbol-13-acetate (TPA) suppressed the expression of the erythrocytic (glycophorin A) and myelocytic (CD11b) antigens in K562-L, but increased the expression of these antigens in K562-H. TPA increased the megakaryocytic (CD61) antigens in both cells. These findings suggest that there are distinct TPA responsible factors in K562-L and -H on the expression of the erythrocytic and myelocytic antigens.
