豚卵胞の顆粒層細胞の走査電子顕微鏡的研究

宮本 元; 古林 亮介; 谷井 隆夫; 石橋 武彦

doi:10.2508/chikusan.53.831

宮本元, 古林亮介, 谷井隆夫, 石橋武彦

著者情報

ジャーナルフリー

1982 年 53 巻 12 号 p. 831-837

DOI https://doi.org/10.2508/chikusan.53.831

詳細

発行日: 1982/12/25 受付日: 1982/07/13 J-STAGE公開日: 2008/03/10 受理日: - 早期公開日: - 改訂日: -
訂正情報
訂正日: 2008/03/10 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1) SHORT, R. V., Recent Progr. Hormone Res., 20: 303-340. 1965.
2) SAVARD, K., J. M. MARSH and B. F. RICE, Recent Progr. Hormone Res., 21: 285-365. 1965.
3) DORFMAN, R. I., in Handbook of Physiology. section 7. vol. 2. part 1. (GREED, R. O. and E. B. ASTDWOOD, eds.) 537-546. Williams and Wilkins Comp. Baltimore. 1973.
4) BJERSING, L., in The Ovary. 2nd ed. Vol. 1. (ZUCKERMAN, L. and B. J. WEIR, eds.) 303-391. Academic Press. New York. 1977.
5) ECKSTEIN, P., in The Ovary. 2nd ed. vol. 2. (ZUCKERMAN, L. and B. J. WEIR, eds.) 275-313. Academic Press. New York. 1977.
6) CHANNING, C. P., F. W. SCHAERF, L. D. ANDERSON and A. TSAFRIRI, Reproductive Physiology. International Review of Physiology. vol. 22 (GREEP, R. O., ed.) 117-201. University Park Press. Baltimore. 1980.
7) STEINETZ, B. G., in Handbook of Physiology. section 7. vol. 2. part 1. (GREEP, R. O. and E. B. ASTWOOD, eds.) 439-466. Williams and Wilkins Comp. Baltimore. 1973.
8) MCNATTY,K. P., in The Vertebrate Ovary. (JONES, R. E. ed.) 215-259. Plenum Press. New York. 1978.
9) CHANG, S. C. S. and R. J. RYAN, Mayo Clinic Proceedings, 51: 621-623. 1976.
10) CHANG, S. C. S., W. ANDERSON, J. C. LEWIS, R. J. RYAN and Y. H. KANG, Biol. Reprod., 16: 349-357. 1977.
11) GWATKIN, R. B. L. and H. W. CARTER, in Scanning Electron Microscopic Atlas of Mammalian Reproduction (HAFEZ, E. S. E. ed.) 270-279. Igaku Shoin Ltd. Tokyo. 1975.
12) HANSEL, W., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 223-265. Academic Press. New York. 1959.
13) BODA, J. M., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 335-357. Academic Press. New York. 1959.
14) HAFEZ, E. S. E., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed). 61-80. Lea and Febiger. Philadelphia. 1968.
15) DAY, B. N., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed.) 279-288. Lea and Febiger. Philadelphia. 1968.
16) CHANNING, C. P. and S. KAMMERMAN, Endocrinology, 92: 531-540. 1973.
17) LEE, C. Y., Endocrinology, 99: 42-48. 1976.
18) RYAN, R. J. and C. Y. LEE, Biol. Reprod., 14: 16-29. 1976.
19) PORTER, K., D. PRESCOTT and J. FRYE, J. Cell Biol., 57: 815-836. 1973.
20) BOYDE, A., R. A. WEISS and P. VESELY, Exp. Cell Res., 71: 313-324. 1972.
21) WILLOCH, M., Acta Pathol. Microbiol. Scand., 71: 35-45. 1967.
22) FOLLETT, E. A. C. and R. D. GOLDMAN, Exp. Cell Res., 59: 124-136. 1970.
23) ANDREW S, P. M. and K. R. PORTER, Anat. Rec., 177: 409-426. 1973.
24) EILER, H. and A. V. NALBANDOV, Endocrinology, 100: 331-338. 1977.

Right : 1) SHORT, R. V., Recent Progr. Hormone Res., 20: 303-340. 1965.
2) SAVARD, K., J. M. MARSH and B. F. RICE, Recent Progr. Hormone Res., 21: 285-365. 1965.
3) DORFMAN, R. I., in Handbook of Physiology. section 7. vol. 2. part 1. (GREED, R. O. and E. B. ASTDWOOD, eds.) 537-546. Williams and Wilkins Comp. Baltimore. 1973.
4) BJERSING, L., in The Ovary. 2nd ed. Vol. 1. (ZUCKERMAN, L. and B. J. WEIR, eds.) 303-391. Academic Press. New York. 1977.
5) ECKSTEIN, P., in The Ovary. 2nd ed. vol. 2. (ZUCKERMAN, L. and B. J. WEIR, eds.) 275-313. Academic Press. New York. 1977.
6) CHANNING, C. P., F. W. SCHAERF, L. D. ANDERSON and A. TSAFRIRI, Reproductive Physiology. International Review of Physiology. vol. 22 (GREEP, R. O., ed.) 117-201. University Park Press. Baltimore. 1980.
7) STEINETZ, B. G., in Handbook of Physiology. section 7. vol. 2. part 1. (GREEP, R. O. and E. B. ASTWOOD, eds.) 439-466. Williams and Wilkins Comp. Baltimore. 1973.
8) MCNATTY,K. P., in The Vertebrate Ovary. (JONES, R. E. ed.) 215-259. Plenum Press. New York. 1978.
9) CHANG, S. C. S. and R. J. RYAN, Mayo Clinic Proceedings, 51: 621-623. 1976.
10) CHANG, S. C. S., W. ANDERSON, J. C. LEWIS, R. J. RYAN and Y. H. KANG, Biol. Reprod., 16: 349-357. 1977.
11) GWATKIN, R. B. L. and H. W. CARTER, in Scanning Electron Microscopic Atlas of Mammalian Reproduction (HAFEZ, E. S. E. ed.) 270-279. Igaku Shoin Ltd. Tokyo. 1975.
12) HANSEL, W., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 223-265. Academic Press. New York. 1959.
13) BODA, J. M., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 335-357. Academic Press. New York. 1959.
14) HAFEZ, E. S. E., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed). 61-80. Lea and Febiger. Philadelphia. 1968.
15) DAY, B. N., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed.) 279-288. Lea and Febiger. Philadelphia. 1968.
16) CHANNING, C. P. and S. KAMMERMAN, Endocrinology, 92: 531-540. 1973.
17) LEE, C. Y., Endocrinology, 99: 42-48. 1976.
18) RYAN, R. J. and C. Y. LEE, Biol. Reprod., 14: 16-29. 1976.
19) PORTER, K., D. PRESCOTT and J. FRYE, J. Cell Biol., 57: 815-836. 1973.
20) BOYDE, A., R. A. WEISS and P. VESELY, Exp. Cell Res., 71: 313-324. 1972.
21) WILLOCH, M., Acta Pathol. Microbiol. Scand., 71: 35-45. 1967.
22) FOLLETT, E. A. C. and R. D. GOLDMAN, Exp. Cell Res., 59: 124-136. 1970.
23) ANDREW S, P. M. and K. R. PORTER, Anat. Rec., 177: 409-426. 1973.
24) EILER, H. and A. V. NALBANDOV, Endocrinology, 100: 331-338. 1977.

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/03/10 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1) SHORT, R. V., Recent Progr. Hormone Res., 20: 303-340. 1965.
2) SAVARD, K., J. M. MARSH and B. F. RICE, Recent Progr. Hormone Res., 21: 285-365. 1965.
3) DORFMAN, R. I., in Handbook of Physiology. section 7. vol. 2. part 1. (GREED, R. O. and E. B. ASTDWOOD, eds.) 537-546. Williams and Wilkins Comp. Baltimore. 1973.
4) BJERSING, L., in The Ovary. 2nd ed. Vol. 1. (ZUCKERMAN, L. and B. J. WEIR, eds.) 303-391. Academic Press. New York. 1977.
5) ECKSTEIN, P., in The Ovary. 2nd ed. vol. 2. (ZUCKERMAN, L. and B. J. WEIR, eds.) 275-313. Academic Press. New York. 1977.
6) CHANNING, C. P., F. W. SCHAERF, L. D. ANDERSON and A. TSAFRIRI, Reproductive Physiology. International Review of Physiology. vol. 22 (GREEP, R. O., ed.) 117-201. University Park Press. Baltimore. 1980.
7) STEINETZ, B. G., in Handbook of Physiology. section 7. vol. 2. part 1. (GREEP, R. O. and E. B. ASTWOOD, eds.) 439-466. Williams and Wilkins Comp. Baltimore. 1973.
8) MCNATTY,K. P., in The Vertebrate Ovary. (JONES, R. E. ed.) 215-259. Plenum Press. New York. 1978.
9) CHANG, S. C. S. and R. J. RYAN, Mayo Clinic Proceedings, 51: 621-623. 1976.
10) CHANG, S. C. S., W. ANDERSON, J. C. LEWIS, R. J. RYAN and Y. H. KANG, Biol. Reprod., 16: 349-357. 1977.
11) GWATKIN, R. B. L. and H. W. CARTER, in Scanning Electron Microscopic Atlas of Mammalian Reproduction (HAFEZ, E. S. E. ed.) 270-279. Igaku Shoin Ltd. Tokyo. 1975.
12) HANSEL, W., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 223-265. Academic Press. New York. 1959.
13) BODA, J. M., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 335-357. Academic Press. New York. 1959.
14) HAFEZ, E. S. E., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed). 61-80. Lea and Febiger. Philadelphia. 1968.
15) DAY, B. N., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed.) 279-288. Lea and Febiger. Philadelphia. 1968.
16) CHANNING, C. P. and S. KAMMERMAN, Endocrinology, 92: 531-540. 1973.
17) LEE, C. Y., Endocrinology, 99: 42-48. 1976.
18) RYAN, R. J. and C. Y. LEE, Biol. Reprod., 14: 16-29. 1976.
19) PORTER, K., D. PRESCOTT and J. FRYE, J. Cell Biol., 57: 815-836. 1973.
20) BOYDE, A., R. A. WEISS and P. VESELY, Exp. Cell Res., 71: 313-324. 1972.
21) WILLOCH, M., Acta Pathol. Microbiol. Scand., 71: 35-45. 1967.
22) FOLLETT, E. A. C. and R. D. GOLDMAN, Exp. Cell Res., 59: 124-136. 1970.
23) ANDREW S, P. M. and K. R. PORTER, Anat. Rec., 177: 409-426. 1973.
24) EILER, H. and A. V. NALBANDOV, Endocrinology, 100: 331-338. 1977.

Right : 1) SHORT, R. V., Recent Progr. Hormone Res., 20: 303-340. 1965.
2) SAVARD, K., J. M. MARSH and B. F. RICE, Recent Progr. Hormone Res., 21: 285-365. 1965.
3) DORFMAN, R. I., in Handbook of Physiology. section 7. vol. 2. part 1. (GREED, R. O. and E. B. ASTDWOOD, eds.) 537-546. Williams and Wilkins Comp. Baltimore. 1973.
4) BJERSING, L., in The Ovary. 2nd ed. Vol. 1. (ZUCKERMAN, L. and B. J. WEIR, eds.) 303-391. Academic Press. New York. 1977.
5) ECKSTEIN, P., in The Ovary. 2nd ed. vol. 2. (ZUCKERMAN, L. and B. J. WEIR, eds.) 275-313. Academic Press. New York. 1977.
6) CHANNING, C. P., F. W. SCHAERF, L. D. ANDERSON and A. TSAFRIRI, Reproductive Physiology. International Review of Physiology. vol. 22 (GREEP, R. O., ed.) 117-201. University Park Press. Baltimore. 1980.
7) STEINETZ, B. G., in Handbook of Physiology. section 7. vol. 2. part 1. (GREEP, R. O. and E. B. ASTWOOD, eds.) 439-466. Williams and Wilkins Comp. Baltimore. 1973.
8) MCNATTY,K. P., in The Vertebrate Ovary. (JONES, R. E. ed.) 215-259. Plenum Press. New York. 1978.
9) CHANG, S. C. S. and R. J. RYAN, Mayo Clinic Proceedings, 51: 621-623. 1976.
10) CHANG, S. C. S., W. ANDERSON, J. C. LEWIS, R. J. RYAN and Y. H. KANG, Biol. Reprod., 16: 349-357. 1977.
11) GWATKIN, R. B. L. and H. W. CARTER, in Scanning Electron Microscopic Atlas of Mammalian Reproduction (HAFEZ, E. S. E. ed.) 270-279. Igaku Shoin Ltd. Tokyo. 1975.
12) HANSEL, W., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 223-265. Academic Press. New York. 1959.
13) BODA, J. M., in Reproduction in Domestic Animals. (COLE, H. H. and P. T. CUPPS, eds.) 335-357. Academic Press. New York. 1959.
14) HAFEZ, E. S. E., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed). 61-80. Lea and Febiger. Philadelphia. 1968.
15) DAY, B. N., in Reproduction in Farm Animals. 2nd ed. (HAFEZ, E. S. E., ed.) 279-288. Lea and Febiger. Philadelphia. 1968.
16) CHANNING, C. P. and S. KAMMERMAN, Endocrinology, 92: 531-540. 1973.
17) LEE, C. Y., Endocrinology, 99: 42-48. 1976.
18) RYAN, R. J. and C. Y. LEE, Biol. Reprod., 14: 16-29. 1976.
19) PORTER, K., D. PRESCOTT and J. FRYE, J. Cell Biol., 57: 815-836. 1973.
20) BOYDE, A., R. A. WEISS and P. VESELY, Exp. Cell Res., 71: 313-324. 1972.
21) WILLOCH, M., Acta Pathol. Microbiol. Scand., 71: 35-45. 1967.
22) FOLLETT, E. A. C. and R. D. GOLDMAN, Exp. Cell Res., 59: 124-136. 1970.
23) ANDREW S, P. M. and K. R. PORTER, Anat. Rec., 177: 409-426. 1973.
24) EILER, H. and A. V. NALBANDOV, Endocrinology, 100: 331-338. 1977.

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

豚卵胞の顆粒層細胞表面の微細構造を明らかにするために,この実験をおこなった.20頭の未成熟または成熟豚の卵巣から,種々の大きさの正常卵胞とのう腫卵胞を採取した,これらの卵胞を分割した後,2.5%グルタルアルデヒドで固定し,1%オスミウム酸で後固定した.ついで臨界点乾燥後,金のイオンスパッタコーティングをおこない,顆粒層細胞を走査電子顕微鏡で観察した.小卵胞(1～2mm)および中卵胞(3～5mm)の顆粒層細胞の表面は比較的なめらかで,多くの場合微絨毛はみられなかった.これに対して,大卵胞(6～12mm)の顆粒層細胞の表画は一般に多くの微絨毛でおおわれ,とくに卵胞腔に近い細胞でこの傾向がみられた.これらの成績から,顆粒層細胞における微絨毛の出現は,卵胞の発育と関係のあることが推察できる.卵胞の大きさが同じであれば,性成熟および発情周期による顆粒層細胞の表面構造の差はほとんど認められなかった.軽いのう腫卵胞には崩壊した顆粒層細胞が存在し,これらの細胞は少数の微絨毛でおおわれていた.一方,進んだのう腫卵胞では顆粒層細胞が消失し,内層には一般に線維素または膠原線維が観察された.

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