ヤギの主要組織適合性遺伝子複合体class II領域の構造とβ鎖遺伝子構成

林 智人; 天野 卓

doi:10.2508/chikusan.65.447

林智人, 天野卓

著者情報

ジャーナルフリー

1994 年 65 巻 5 号 p. 447-453

DOI https://doi.org/10.2508/chikusan.65.447

詳細

発行日: 1994/05/25 受付日: 1993/09/06 J-STAGE公開日: 2008/03/10 受理日: - 早期公開日: - 改訂日: -
訂正情報
訂正日: 2008/03/10 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1) ANDERSSON, L., A. LUNDEN, S. SIGURDARDOTTIR, C. J. DAVIES and L. RASK, Linkage relationships in the bovine MHC region. High recombination frequency between class II subregions. Immunogenetics, 27: 273-280. 1988.
2) BELL, G. I., J. H. KARAM and W. J. RUTTER, Polymorphic DNA region adjacent to the 5' end of the human insulin gene. Proc. Natl. Acad. Sci. U. S. A., 78: 5759-5763. 1981.
3) CAMERON, P. U., H. A. TABARIAS, B. PULENDRAN, W. ROBINSON and R. L. DAWKINS, Conservation of the MHC genome: PFGE mapping and RFLP analysis of complement, HSP70, and TNF genes in the goat. Immunogenetics, 31: 253-264. 1990.
4) DEVERSON, E. V., H. WRIGHT, S. WATSON, K. BALLINGALL, N. HUSKISSON, A. G. DIAMOND and J. C. HOWARD, Class II major histocompatibility complex genes of the sheep. Anim. Genet., 22: 211-225. 1991.
5) HARDY, D. A., J. I. BELL, E. O. LONG, T. LINDSTEN and H. O. MGDEVITT, Mapping of the class II region of the human major histocompatibility complex by pulsed-field gel electrophoresis. Nature, 323: 453-455. 1986.
6) HAYASHI, T., T. AMANO, F. OTANI and N. KASHIWAGI, Restriction fragment length polymorphism analysis of major histocompatibility complex class II DRβ genes of goats. Anim. Genet., 23 (Supplement 1): 44. 1992.
7) HEDRICK, P., S. JAIN and L. HOLDEN, Multilocus systems in evolution. Evol. Biol., 11: 101-184. 1978.
8) HILL, A. V. S., C. E. M. ALLSOPP, D. KWLATKOWSKI, N. M. ANSTEY, P. TWUMASI, P. A. ROWE, S. BENNETT, D. BREWSTER, A. J. MCMICHAEL and B. M. GREENWOOD, Common West African HLA antigens are associated with protection from severe malaria. Nature, 352: 595-600, 1991.
9) JOOSTEN, I., G. RUFF, M.F. SANDERS and E. J. HENSEN, Use of isoelectric focusing to define major histocompatibility complex class I polymorphism in goats. Anim. Genet., 24: 47-51. 1993.
10) KLEIN, J., F. FIGUEROA and Z. A. NAGY, Genetics of the major histocompatibility complex: the final act. Annu. Rev. Immunol., 1: 119-142. 1983.
11) KOBORI, J. A., E. STRAUSS, K. MINARD and L. HOOD, Molecular analysis of the hotspot of recombination in the murine major histocompatibility complex. Science, 234: 173-179. 1986.
12) KURAGAKI, I., Y. YAMAMOTO, M. MIZUTANI and I. OKADA, Analysis of class IV genes of the chicken major histocompatibility complex using restriction fragment length polymorphisms. Anim. Sci. Technol. (Jpn.), 62: 330-335. 1991.
13) MUGGLI-COCKETT, N. E. and R. T. STONE, Identification of genetic variation in the bovine major histocompatibility complex DRβ-like genes using sequenced bovine genomic probes. Anim. Genet.,19: 213-225. 1988.
14) MUGGLI-COCKETT, N. E. and R. T. STONE, Restriction fragment length polymorphisms in bovine major histocompatibility complex class II β-chain genes using bovine exon-containing hybridization probes. Anim. Genet., 22: 123-136. 1991.
15) NESSE, L. L., G. PAULSEN, M. SYED and G. RUFF, A human major histocompatibility complex (MHC) DNA probe recognizes goat genes. Acta Vet. Scand., 29: 193-198. 1988.
16) NESSE, L. L. and G. RUFF, A comparison of lymphocyte antigen specificities in Norwegian and Swiss goats. Anim. Genet., 20: 71-77. 1989.
17) PERARNAU, B., H. GOURNIER, F. A. LEMONNIER, C. BARRA and R. HAKEM, Structure of antigens (VAN REGENMMORTL, M. H. V., eds.). 1st ed. 339-366. CRC Press. Florida. 1992.
18) RILEY, E. M., O. OLERUP, S. BENNETT, P. ROWE, S. J. ALLENN, M. J. BLACKMAN, M. TROYEBLOMBERG, A. A. HOLDER and B.M. GREENWOOD, MHC and malaria: the relationship between HLA class II alleles and immune responses to Plasmodium falciparum. Int. Immunol., 4: 1055-1063. 1992.
19) STEINMETZ, M., D. STEPHAN and K. FISCHERLINDAHI, Gene organization and recombination hotspots in the murine major histocompatibility complex. Cell, 44: 895-904. 1986.
20) STONE, R. T., and N. E. MUGGLI-COCKETT, Partial nucleotide sequence of a novel bovine major histocompatibility complex class II β-chain gene, BOLA-DIB. Anim. Genet.,21: 353-360. 1990.
21) STONE, R. T., and N. E. MUGGLI-COCKETT, BOLADIB: species distribution, linkage with DOB, and Northern analysis. Anim. Genet., 24: 41-45. 1993.
22) TEALE, A. J., S. J. KEMP and W. I. MORRISON, Breeding for disease resistance in farm animal (OWEN, J. B. and R. F. E. AXFORD, eds.). 1st ed. 86-99. CAB International. Wallingford, 1991.
23) TERMIJTELEN, A., P. MEERA KHAN, S. SHAW and J. J. VAN ROOD, Mapping SB in relation to HLA and GLOl using cells from first-cousin marriage offspring. Immunogenetics, 18: 503-512. 1983.
24) VAN DAM, R. H., J. D'AMARO, P. J. S. VAN KOOTEN, J. A. VAN DER DONK and J. GOUDSWAARD, The histocompatibility complex GLA in the goat. Anim. Blood Grps. Biochem. Genet., 10: 121-124. 1979.

Right : 1) ANDERSSON, L., A. LUNDEN, S. SIGURDARDOTTIR, C. J. DAVIES and L. RASK, Linkage relationships in the bovine MHC region. High recombination frequency between class II subregions. Immunogenetics, 27: 273-280. 1988.
2) BELL, G. I., J. H. KARAM and W. J. RUTTER, Polymorphic DNA region adjacent to the 5' end of the human insulin gene. Proc. Natl. Acad. Sci. U. S. A., 78: 5759-5763. 1981.
3) CAMERON, P. U., H. A. TABARIAS, B. PULENDRAN, W. ROBINSON and R. L. DAWKINS, Conservation of the MHC genome: PFGE mapping and RFLP analysis of complement, HSP70, and TNF genes in the goat. Immunogenetics, 31: 253-264. 1990.
4) DEVERSON, E. V., H. WRIGHT, S. WATSON, K. BALLINGALL, N. HUSKISSON, A. G. DIAMOND and J. C. HOWARD, Class II major histocompatibility complex genes of the sheep. Anim. Genet., 22: 211-225. 1991.
5) HARDY, D. A., J. I. BELL, E. O. LONG, T. LINDSTEN and H. O. MGDEVITT, Mapping of the class II region of the human major histocompatibility complex by pulsed-field gel electrophoresis. Nature, 323: 453-455. 1986.
6) HAYASHI, T., T. AMANO, F. OTANI and N. KASHIWAGI, Restriction fragment length polymorphism analysis of major histocompatibility complex class II DRβ genes of goats. Anim. Genet., 23 (Supplement 1): 44. 1992.
7) HEDRICK, P., S. JAIN and L. HOLDEN, Multilocus systems in evolution. Evol. Biol., 11: 101-184. 1978.
8) HILL, A. V. S., C. E. M. ALLSOPP, D. KWLATKOWSKI, N. M. ANSTEY, P. TWUMASI, P. A. ROWE, S. BENNETT, D. BREWSTER, A. J. MCMICHAEL and B. M. GREENWOOD, Common West African HLA antigens are associated with protection from severe malaria. Nature, 352: 595-600, 1991.
9) JOOSTEN, I., G. RUFF, M.F. SANDERS and E. J. HENSEN, Use of isoelectric focusing to define major histocompatibility complex class I polymorphism in goats. Anim. Genet., 24: 47-51. 1993.
10) KLEIN, J., F. FIGUEROA and Z. A. NAGY, Genetics of the major histocompatibility complex: the final act. Annu. Rev. Immunol., 1: 119-142. 1983.
11) KOBORI, J. A., E. STRAUSS, K. MINARD and L. HOOD, Molecular analysis of the hotspot of recombination in the murine major histocompatibility complex. Science, 234: 173-179. 1986.
12) KURAGAKI, I., Y. YAMAMOTO, M. MIZUTANI and I. OKADA, Analysis of class IV genes of the chicken major histocompatibility complex using restriction fragment length polymorphisms. Anim. Sci. Technol. (Jpn.), 62: 330-335. 1991.
13) MUGGLI-COCKETT, N. E. and R. T. STONE, Identification of genetic variation in the bovine major histocompatibility complex DRβ-like genes using sequenced bovine genomic probes. Anim. Genet.,19: 213-225. 1988.
14) MUGGLI-COCKETT, N. E. and R. T. STONE, Restriction fragment length polymorphisms in bovine major histocompatibility complex class II β-chain genes using bovine exon-containing hybridization probes. Anim. Genet., 22: 123-136. 1991.
15) NESSE, L. L., G. PAULSEN, M. SYED and G. RUFF, A human major histocompatibility complex (MHC) DNA probe recognizes goat genes. Acta Vet. Scand., 29: 193-198. 1988.
16) NESSE, L. L. and G. RUFF, A comparison of lymphocyte antigen specificities in Norwegian and Swiss goats. Anim. Genet., 20: 71-77. 1989.
17) PERARNAU, B., H. GOURNIER, F. A. LEMONNIER, C. BARRA and R. HAKEM, Structure of antigens (VAN REGENMMORTL, M. H. V., eds.). 1st ed. 339-366. CRC Press. Florida. 1992.
18) RILEY, E. M., O. OLERUP, S. BENNETT, P. ROWE, S. J. ALLENN, M. J. BLACKMAN, M. TROYEBLOMBERG, A. A. HOLDER and B.M. GREENWOOD, MHC and malaria: the relationship between HLA class II alleles and immune responses to Plasmodium falciparum. Int. Immunol., 4: 1055-1063. 1992.
19) STEINMETZ, M., D. STEPHAN and K. FISCHERLINDAHI, Gene organization and recombination hotspots in the murine major histocompatibility complex. Cell, 44: 895-904. 1986.
20) STONE, R. T., and N. E. MUGGLI-COCKETT, Partial nucleotide sequence of a novel bovine major histocompatibility complex class II β-chain gene, BOLA-DIB. Anim. Genet.,21: 353-360. 1990.
21) STONE, R. T., and N. E. MUGGLI-COCKETT, BOLADIB: species distribution, linkage with DOB, and Northern analysis. Anim. Genet., 24: 41-45. 1993.
22) TEALE, A. J., S. J. KEMP and W. I. MORRISON, Breeding for disease resistance in farm animal (OWEN, J. B. and R. F. E. AXFORD, eds.). 1st ed. 86-99. CAB International. Wallingford, 1991.
23) TERMIJTELEN, A., P. MEERA KHAN, S. SHAW and J. J. VAN ROOD, Mapping SB in relation to HLA and GLOl using cells from first-cousin marriage offspring. Immunogenetics, 18: 503-512. 1983.
24) VAN DAM, R. H., J. D'AMARO, P. J. S. VAN KOOTEN, J. A. VAN DER DONK and J. GOUDSWAARD, The histocompatibility complex GLA in the goat. Anim. Blood Grps. Biochem. Genet., 10: 121-124. 1979.

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/03/10 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1) ANDERSSON, L., A. LUNDEN, S. SIGURDARDOTTIR, C. J. DAVIES and L. RASK, Linkage relationships in the bovine MHC region. High recombination frequency between class II subregions. Immunogenetics, 27: 273-280. 1988.
2) BELL, G. I., J. H. KARAM and W. J. RUTTER, Polymorphic DNA region adjacent to the 5' end of the human insulin gene. Proc. Natl. Acad. Sci. U. S. A., 78: 5759-5763. 1981.
3) CAMERON, P. U., H. A. TABARIAS, B. PULENDRAN, W. ROBINSON and R. L. DAWKINS, Conservation of the MHC genome: PFGE mapping and RFLP analysis of complement, HSP70, and TNF genes in the goat. Immunogenetics, 31: 253-264. 1990.
4) DEVERSON, E. V., H. WRIGHT, S. WATSON, K. BALLINGALL, N. HUSKISSON, A. G. DIAMOND and J. C. HOWARD, Class II major histocompatibility complex genes of the sheep. Anim. Genet., 22: 211-225. 1991.
5) HARDY, D. A., J. I. BELL, E. O. LONG, T. LINDSTEN and H. O. MGDEVITT, Mapping of the class II region of the human major histocompatibility complex by pulsed-field gel electrophoresis. Nature, 323: 453-455. 1986.
6) HAYASHI, T., T. AMANO, F. OTANI and N. KASHIWAGI, Restriction fragment length polymorphism analysis of major histocompatibility complex class II DRβ genes of goats. Anim. Genet., 23 (Supplement 1): 44. 1992.
7) HEDRICK, P., S. JAIN and L. HOLDEN, Multilocus systems in evolution. Evol. Biol., 11: 101-184. 1978.
8) HILL, A. V. S., C. E. M. ALLSOPP, D. KWLATKOWSKI, N. M. ANSTEY, P. TWUMASI, P. A. ROWE, S. BENNETT, D. BREWSTER, A. J. MCMICHAEL and B. M. GREENWOOD, Common West African HLA antigens are associated with protection from severe malaria. Nature, 352: 595-600, 1991.
9) JOOSTEN, I., G. RUFF, M.F. SANDERS and E. J. HENSEN, Use of isoelectric focusing to define major histocompatibility complex class I polymorphism in goats. Anim. Genet., 24: 47-51. 1993.
10) KLEIN, J., F. FIGUEROA and Z. A. NAGY, Genetics of the major histocompatibility complex: the final act. Annu. Rev. Immunol., 1: 119-142. 1983.
11) KOBORI, J. A., E. STRAUSS, K. MINARD and L. HOOD, Molecular analysis of the hotspot of recombination in the murine major histocompatibility complex. Science, 234: 173-179. 1986.
12) KURAGAKI, I., Y. YAMAMOTO, M. MIZUTANI and I. OKADA, Analysis of class IV genes of the chicken major histocompatibility complex using restriction fragment length polymorphisms. Anim. Sci. Technol. (Jpn.), 62: 330-335. 1991.
13) MUGGLI-COCKETT, N. E. and R. T. STONE, Identification of genetic variation in the bovine major histocompatibility complex DRβ-like genes using sequenced bovine genomic probes. Anim. Genet.,19: 213-225. 1988.
14) MUGGLI-COCKETT, N. E. and R. T. STONE, Restriction fragment length polymorphisms in bovine major histocompatibility complex class II β-chain genes using bovine exon-containing hybridization probes. Anim. Genet., 22: 123-136. 1991.
15) NESSE, L. L., G. PAULSEN, M. SYED and G. RUFF, A human major histocompatibility complex (MHC) DNA probe recognizes goat genes. Acta Vet. Scand., 29: 193-198. 1988.
16) NESSE, L. L. and G. RUFF, A comparison of lymphocyte antigen specificities in Norwegian and Swiss goats. Anim. Genet., 20: 71-77. 1989.
17) PERARNAU, B., H. GOURNIER, F. A. LEMONNIER, C. BARRA and R. HAKEM, Structure of antigens (VAN REGENMMORTL, M. H. V., eds.). 1st ed. 339-366. CRC Press. Florida. 1992.
18) RILEY, E. M., O. OLERUP, S. BENNETT, P. ROWE, S. J. ALLENN, M. J. BLACKMAN, M. TROYEBLOMBERG, A. A. HOLDER and B.M. GREENWOOD, MHC and malaria: the relationship between HLA class II alleles and immune responses to Plasmodium falciparum. Int. Immunol., 4: 1055-1063. 1992.
19) STEINMETZ, M., D. STEPHAN and K. FISCHERLINDAHI, Gene organization and recombination hotspots in the murine major histocompatibility complex. Cell, 44: 895-904. 1986.
20) STONE, R. T., and N. E. MUGGLI-COCKETT, Partial nucleotide sequence of a novel bovine major histocompatibility complex class II β-chain gene, BOLA-DIB. Anim. Genet.,21: 353-360. 1990.
21) STONE, R. T., and N. E. MUGGLI-COCKETT, BOLADIB: species distribution, linkage with DOB, and Northern analysis. Anim. Genet., 24: 41-45. 1993.
22) TEALE, A. J., S. J. KEMP and W. I. MORRISON, Breeding for disease resistance in farm animal (OWEN, J. B. and R. F. E. AXFORD, eds.). 1st ed. 86-99. CAB International. Wallingford, 1991.
23) TERMIJTELEN, A., P. MEERA KHAN, S. SHAW and J. J. VAN ROOD, Mapping SB in relation to HLA and GLOl using cells from first-cousin marriage offspring. Immunogenetics, 18: 503-512. 1983.
24) VAN DAM, R. H., J. D'AMARO, P. J. S. VAN KOOTEN, J. A. VAN DER DONK and J. GOUDSWAARD, The histocompatibility complex GLA in the goat. Anim. Blood Grps. Biochem. Genet., 10: 121-124. 1979.

Right : 1) ANDERSSON, L., A. LUNDEN, S. SIGURDARDOTTIR, C. J. DAVIES and L. RASK, Linkage relationships in the bovine MHC region. High recombination frequency between class II subregions. Immunogenetics, 27: 273-280. 1988.
2) BELL, G. I., J. H. KARAM and W. J. RUTTER, Polymorphic DNA region adjacent to the 5' end of the human insulin gene. Proc. Natl. Acad. Sci. U. S. A., 78: 5759-5763. 1981.
3) CAMERON, P. U., H. A. TABARIAS, B. PULENDRAN, W. ROBINSON and R. L. DAWKINS, Conservation of the MHC genome: PFGE mapping and RFLP analysis of complement, HSP70, and TNF genes in the goat. Immunogenetics, 31: 253-264. 1990.
4) DEVERSON, E. V., H. WRIGHT, S. WATSON, K. BALLINGALL, N. HUSKISSON, A. G. DIAMOND and J. C. HOWARD, Class II major histocompatibility complex genes of the sheep. Anim. Genet., 22: 211-225. 1991.
5) HARDY, D. A., J. I. BELL, E. O. LONG, T. LINDSTEN and H. O. MGDEVITT, Mapping of the class II region of the human major histocompatibility complex by pulsed-field gel electrophoresis. Nature, 323: 453-455. 1986.
6) HAYASHI, T., T. AMANO, F. OTANI and N. KASHIWAGI, Restriction fragment length polymorphism analysis of major histocompatibility complex class II DRβ genes of goats. Anim. Genet., 23 (Supplement 1): 44. 1992.
7) HEDRICK, P., S. JAIN and L. HOLDEN, Multilocus systems in evolution. Evol. Biol., 11: 101-184. 1978.
8) HILL, A. V. S., C. E. M. ALLSOPP, D. KWLATKOWSKI, N. M. ANSTEY, P. TWUMASI, P. A. ROWE, S. BENNETT, D. BREWSTER, A. J. MCMICHAEL and B. M. GREENWOOD, Common West African HLA antigens are associated with protection from severe malaria. Nature, 352: 595-600, 1991.
9) JOOSTEN, I., G. RUFF, M.F. SANDERS and E. J. HENSEN, Use of isoelectric focusing to define major histocompatibility complex class I polymorphism in goats. Anim. Genet., 24: 47-51. 1993.
10) KLEIN, J., F. FIGUEROA and Z. A. NAGY, Genetics of the major histocompatibility complex: the final act. Annu. Rev. Immunol., 1: 119-142. 1983.
11) KOBORI, J. A., E. STRAUSS, K. MINARD and L. HOOD, Molecular analysis of the hotspot of recombination in the murine major histocompatibility complex. Science, 234: 173-179. 1986.
12) KURAGAKI, I., Y. YAMAMOTO, M. MIZUTANI and I. OKADA, Analysis of class IV genes of the chicken major histocompatibility complex using restriction fragment length polymorphisms. Anim. Sci. Technol. (Jpn.), 62: 330-335. 1991.
13) MUGGLI-COCKETT, N. E. and R. T. STONE, Identification of genetic variation in the bovine major histocompatibility complex DRβ-like genes using sequenced bovine genomic probes. Anim. Genet.,19: 213-225. 1988.
14) MUGGLI-COCKETT, N. E. and R. T. STONE, Restriction fragment length polymorphisms in bovine major histocompatibility complex class II β-chain genes using bovine exon-containing hybridization probes. Anim. Genet., 22: 123-136. 1991.
15) NESSE, L. L., G. PAULSEN, M. SYED and G. RUFF, A human major histocompatibility complex (MHC) DNA probe recognizes goat genes. Acta Vet. Scand., 29: 193-198. 1988.
16) NESSE, L. L. and G. RUFF, A comparison of lymphocyte antigen specificities in Norwegian and Swiss goats. Anim. Genet., 20: 71-77. 1989.
17) PERARNAU, B., H. GOURNIER, F. A. LEMONNIER, C. BARRA and R. HAKEM, Structure of antigens (VAN REGENMMORTL, M. H. V., eds.). 1st ed. 339-366. CRC Press. Florida. 1992.
18) RILEY, E. M., O. OLERUP, S. BENNETT, P. ROWE, S. J. ALLENN, M. J. BLACKMAN, M. TROYEBLOMBERG, A. A. HOLDER and B.M. GREENWOOD, MHC and malaria: the relationship between HLA class II alleles and immune responses to Plasmodium falciparum. Int. Immunol., 4: 1055-1063. 1992.
19) STEINMETZ, M., D. STEPHAN and K. FISCHERLINDAHI, Gene organization and recombination hotspots in the murine major histocompatibility complex. Cell, 44: 895-904. 1986.
20) STONE, R. T., and N. E. MUGGLI-COCKETT, Partial nucleotide sequence of a novel bovine major histocompatibility complex class II β-chain gene, BOLA-DIB. Anim. Genet.,21: 353-360. 1990.
21) STONE, R. T., and N. E. MUGGLI-COCKETT, BOLADIB: species distribution, linkage with DOB, and Northern analysis. Anim. Genet., 24: 41-45. 1993.
22) TEALE, A. J., S. J. KEMP and W. I. MORRISON, Breeding for disease resistance in farm animal (OWEN, J. B. and R. F. E. AXFORD, eds.). 1st ed. 86-99. CAB International. Wallingford, 1991.
23) TERMIJTELEN, A., P. MEERA KHAN, S. SHAW and J. J. VAN ROOD, Mapping SB in relation to HLA and GLOl using cells from first-cousin marriage offspring. Immunogenetics, 18: 503-512. 1983.
24) VAN DAM, R. H., J. D'AMARO, P. J. S. VAN KOOTEN, J. A. VAN DER DONK and J. GOUDSWAARD, The histocompatibility complex GLA in the goat. Anim. Blood Grps. Biochem. Genet., 10: 121-124. 1979.

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

ヤギの主要組織適合性遺伝子複合体(MHC) class II領域の構造とβ鎖遺伝子構成を把握するため,ウシclass II遺伝子のDRB, DQBおよびDIB遺伝子座を特異的に認識するR2-TM, B1-TMおよびI1-TMをプローブとしたRFLP解析を行なった.用いた3種のプローブ間では,同一サザンブロット上クロスハイブリダイゼーションは検出されず,各プローブがそれぞれ独立した遺伝子領域を認
識していることが判明した.また各プローブは,R2-TM/Taq Iで4.4-,3.3-および2.7-kbの3種,B1-TM/Pvu IIで4.8-および2.1-kbの2種,I1-TM/Taq Iで10.2-, 9.7-および7.6-kbの3種のフラグメントを検出した.これらのフラグメントは,いずれの組合せにおいても共優性であり,それぞれ同一遺伝子座に属する対立遺伝子を認識していることが示された.親子関係にある21頭を用い,3種の遺伝子座間の連鎖関係を調査したところ,DRBとDQB遺伝子座間においては完全な連鎖関係が認められた.また完全連鎖関係にあるDRB/DQBの2遺伝子座とDIB遺伝子座の間では組換えが起きており,その頻度は0.143であった.DRB/DQB遺伝子座間における4.4/4.8, 3.3/2.1および2.7/4.8の3種の対立遺伝子の組合せは,連鎖不平衡状態にあることが観察され,両親からハプロタイプとして遺伝していることが明らかになった.これらのことから,本ヤギ集団におけるclass II領域の構造は,DRBとDQB遺伝子座を含むグループと,DIB遺伝子座を含むグループの2つより構成され,染色体上少なくとも14.3cMの大きさを有していることが推察された.さらに,その2つのグループに挟まれた領域に高頻度で組換えの起るホットスポットの存在が示唆された.

責任著者(Corresponding author)

訂正情報

J-STAGEへの登録はこちら（無料）