RT-PCR-RFLP法を用いた各種ブタ品種におけるSLAクラスII遺伝子のDNAタイピング

小松 正憲; 野村 友宏; 丸野 弘幸; 川上 和夫; マティアス マナエップジョブ

doi:10.2508/chikusan.71.7_1

小松正憲, 野村友宏, 丸野弘幸, 川上和夫, マティアスマナエップジョブ

著者情報

ジャーナルフリー

2000 年 71 巻 7 号 p. 1-11

DOI https://doi.org/10.2508/chikusan.71.7_1

詳細

発行日: 2000/02/25 受付日: 1999/05/26 J-STAGE公開日: 2008/03/10 受理日: 1999/12/15 早期公開日: - 改訂日: -
訂正情報
訂正日: 2008/03/10 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1) Burnsberg U, Edfors-Lilja I, Andersson L, Gustafsson K. Structure and organization of pig MHC class II DRBgenes: evidence for genetic exchange between loci. Immunogenetics, 44: 1-8. 1996.
3) Conley AJ, Jung YC, Schwartz NK, Warner CM, Rothschild MF, Ford SP. Influence of SLA haplotype on ovulation rate and litter size in miniature pigs. Journal of Reproduction and Fertility, 82: 595-601. 1988.
4) Ford SP, Schwartz NK, Rothschild MF, Conley AJ, Warner CM. Influence of SLA haplotype on preimplantation embryonic cell number in miniature pigs. Journal of Reproduction and Fertility, 84: 99-104. 1988.
5) Gustafsson K, LeGuern C, Hirsch F, Germana S, Pratt K, Sachs DH. Class II genes of miniature swine. IV. Charac-terization and expres-sion of two allelic class II DQB cDNA clones. Journal of Immunology, 145: 1946-1951. 1990.
6) Gustafsson K, Germana S, Hirsch F, Pratt K, LeGuern C, Sachs DH. Structure of miniature swine class II DRBgenes: conservation of hypervariable amino acid residues between distantly related mammalian species. Proceedingsof the National Academy of Sciences, USA, 87: 9798-9802. 1990.
7) Hirsch F, Sachs DH, Gustafsson K, Pratt K, Germana S, LeGuern C. Class II genes of miniature swine. III. haracerization of an expressed pig class II gene homologous to HLA-DQA. Immunogenetics, 31: 52-56. 1990.
8) Hirsch F, Germana S, Gustafsson K, Pratt K, Sachs DH, LeGuern C. Structure and expression of Class II α genes in miniature swine. Journal of Immunology, 149: 841-846. 1992.
9) Hughes AL, Nei M. Nucleotide substitution at the major histocompatibility complex II loci: Evidence for over-dominant selection. Proceedings of the National Academy of Sciences, USA, 86: 958-962. 1989.
11) Klein J. Natural History of the Major Histocompatibility Complex. 423-607. John Wiley & Sons, Inc. New York.1986.
13) Lacey C, Wilkie BN, Kennedy BW, Mallard BA. Genetic and other effects on bacterial phagocytosis and killing bycultured peripheral blood monocytes of SLA-defined miniature pigs. Animal Genetics, 20: 371-382. 1989.
14) Lumsden JS, Kennedy BW, Mallard BA, Wilkie BN. The influence of the swine major histocompatibility genes onantibody and cell mediated immune responses to immunization with an aromatic-dependent mutant of Salmonellatyphimurium. Canadian Journal of Veterinary Research, 57: 14-18. 1993.
15) Lunney JK, Pescovitz MD, Sachs DH. The swine major histocompati-bility complex: its structure and function.In: Swine in Biomedical Research vol. 3. (Tumbleson ME ed.) 1821-1835. Plenum Press. New York. 1986.
16) Madden KB, Moeller RF, Douglass LW, Goldman T, Lunney JK. Trichinella spiralis: Genetic basis and kineticsof the anti-encrysted muscle larval response in miniature swine. Experimental Parasitology, 77: 23-25. 1993.
17) Mallard BA, Wilkie BN, Kennedy BW. Genetic and other effects on antibody and cell mediated immune response inswine leucocyte antigen (SLA )-defined miniature pigs. Animal Genetics, 20: 167-178. 1989.
18) Mallard BA, Wilkie BN, Kennedy BW. The influence of the swine major histocompatibility genes (SLA) on varia-tion in serum immunoglobulin (Ig) concentration. Veterinary Immunology and Immunopathology, 21: 139-151. 1989.
19) Nielsen VH, Thomsen B. Pvu II RFLPs at SLA class II loci: DQA and DRA. Animal Genetics, 28: 151. 1997.
20) Peigiong L. Xiang pig: A superior raw material for special pork products (A review). Proceedings of the International Symposium on Chinese Pig Breeds: 525-529. 1992.
21) Prather RS, Gibson J, Parry TW, Hoffman KE. Reproductive measurements in Sinclair and NIH miniature pigs: aretrospective analysis. Theriogenology, 47: 433-440. 1997.
22) Renard C, Vaiman M. Possible relationships between SLA and porcine reproduction. Reproduction, Nutrition and Development, 29: 569-576. 1989.
23) Rothschild MF, Chen HL, Christian LL, Lie WR, Venier L, Cooper M, Briggs C, Warner CM. Breed and winelymphocyte antigen haplotype differences in agglutination titers following vaccination with B. Bronchiseptica. Journal of Animal Science, 59: 643-649. 1984.
24) Shia YC, Bradshaw M, Rutherford MS, Lewin HA, Schook LB. Polymerase chain reaction based genotyping for char-acterization of SLA-DQB and SLA-DRB alleles in domestic pigs. Animal Genetics, 26: 91-100. 1995.
25) Tanaka K, Oishi T, Kurosawa Y, Suzuki S. Genetic relationship among several pig populations in East Asiaanalysed by blood groups and serum protein polymorphism. Animal Blood Groups and Biochemical Genetics, 14: 191-200. 1983.
26) Vage DI, Olsaker I, Lingaas F, Lie O. Isolation and sequence determination of porcine class II DRB allelesamplified by PCR. Animal Genetics, 25: 73-75. 1994.
27) Vaiman M, Metzger J, Renard C, Vila J. Immune response gene (s) controlling the humoral anti-lysozyme response(Ir-Lys) linked to the major histocompatibility complex SLA in the pig. Immunogenetics, 7: 231-238. 1978.
28) Zhang X, Rutherford MS, Shook LB. Characterization of SLA-DRB and SLA-DQB alleles distinguished by PCR-RFLP. Proceeding of XXVth International Conference on Animal Genetics: 85. 1996.

Right : 1) Burnsberg U, Edfors-Lilja I, Andersson L, Gustafsson K. Structure and organization of pig MHC class II DRBgenes: evidence for genetic exchange between loci. Immunogenetics, 44: 1-8. 1996.
3) Conley AJ, Jung YC, Schwartz NK, Warner CM, Rothschild MF, Ford SP. Influence of SLA haplotype on ovulation rate and litter size in miniature pigs. Journal of Reproduction and Fertility, 82: 595-601. 1988.
4) Ford SP, Schwartz NK, Rothschild MF, Conley AJ, Warner CM. Influence of SLA haplotype on preimplantation embryonic cell number in miniature pigs. Journal of Reproduction and Fertility, 84: 99-104. 1988.
5) Gustafsson K, LeGuern C, Hirsch F, Germana S, Pratt K, Sachs DH. Class II genes of miniature swine. IV. Charac-terization and expres-sion of two allelic class II DQB cDNA clones. Journal of Immunology, 145: 1946-1951. 1990.
6) Gustafsson K, Germana S, Hirsch F, Pratt K, LeGuern C, Sachs DH. Structure of miniature swine class II DRBgenes: conservation of hypervariable amino acid residues between distantly related mammalian species. Proceedingsof the National Academy of Sciences, USA, 87: 9798-9802. 1990.
7) Hirsch F, Sachs DH, Gustafsson K, Pratt K, Germana S, LeGuern C. Class II genes of miniature swine. III. haracerization of an expressed pig class II gene homologous to HLA-DQA. Immunogenetics, 31: 52-56. 1990.
8) Hirsch F, Germana S, Gustafsson K, Pratt K, Sachs DH, LeGuern C. Structure and expression of Class II α genes in miniature swine. Journal of Immunology, 149: 841-846. 1992.
9) Hughes AL, Nei M. Nucleotide substitution at the major histocompatibility complex II loci: Evidence for over-dominant selection. Proceedings of the National Academy of Sciences, USA, 86: 958-962. 1989.
11) Klein J. Natural History of the Major Histocompatibility Complex. 423-607. John Wiley & Sons, Inc. New York.1986.
13) Lacey C, Wilkie BN, Kennedy BW, Mallard BA. Genetic and other effects on bacterial phagocytosis and killing bycultured peripheral blood monocytes of SLA-defined miniature pigs. Animal Genetics, 20: 371-382. 1989.
14) Lumsden JS, Kennedy BW, Mallard BA, Wilkie BN. The influence of the swine major histocompatibility genes onantibody and cell mediated immune responses to immunization with an aromatic-dependent mutant of Salmonellatyphimurium. Canadian Journal of Veterinary Research, 57: 14-18. 1993.
15) Lunney JK, Pescovitz MD, Sachs DH. The swine major histocompati-bility complex: its structure and function.In: Swine in Biomedical Research vol. 3. (Tumbleson ME ed.) 1821-1835. Plenum Press. New York. 1986.
16) Madden KB, Moeller RF, Douglass LW, Goldman T, Lunney JK. Trichinella spiralis: Genetic basis and kineticsof the anti-encrysted muscle larval response in miniature swine. Experimental Parasitology, 77: 23-25. 1993.
17) Mallard BA, Wilkie BN, Kennedy BW. Genetic and other effects on antibody and cell mediated immune response inswine leucocyte antigen (SLA )-defined miniature pigs. Animal Genetics, 20: 167-178. 1989.
18) Mallard BA, Wilkie BN, Kennedy BW. The influence of the swine major histocompatibility genes (SLA) on varia-tion in serum immunoglobulin (Ig) concentration. Veterinary Immunology and Immunopathology, 21: 139-151. 1989.
19) Nielsen VH, Thomsen B. Pvu II RFLPs at SLA class II loci: DQA and DRA. Animal Genetics, 28: 151. 1997.
20) Peigiong L. Xiang pig: A superior raw material for special pork products (A review). Proceedings of the International Symposium on Chinese Pig Breeds: 525-529. 1992.
21) Prather RS, Gibson J, Parry TW, Hoffman KE. Reproductive measurements in Sinclair and NIH miniature pigs: aretrospective analysis. Theriogenology, 47: 433-440. 1997.
22) Renard C, Vaiman M. Possible relationships between SLA and porcine reproduction. Reproduction, Nutrition and Development, 29: 569-576. 1989.
23) Rothschild MF, Chen HL, Christian LL, Lie WR, Venier L, Cooper M, Briggs C, Warner CM. Breed and winelymphocyte antigen haplotype differences in agglutination titers following vaccination with B. Bronchiseptica. Journal of Animal Science, 59: 643-649. 1984.
24) Shia YC, Bradshaw M, Rutherford MS, Lewin HA, Schook LB. Polymerase chain reaction based genotyping for char-acterization of SLA-DQB and SLA-DRB alleles in domestic pigs. Animal Genetics, 26: 91-100. 1995.
25) Tanaka K, Oishi T, Kurosawa Y, Suzuki S. Genetic relationship among several pig populations in East Asiaanalysed by blood groups and serum protein polymorphism. Animal Blood Groups and Biochemical Genetics, 14: 191-200. 1983.
26) Vage DI, Olsaker I, Lingaas F, Lie O. Isolation and sequence determination of porcine class II DRB allelesamplified by PCR. Animal Genetics, 25: 73-75. 1994.
27) Vaiman M, Metzger J, Renard C, Vila J. Immune response gene (s) controlling the humoral anti-lysozyme response(Ir-Lys) linked to the major histocompatibility complex SLA in the pig. Immunogenetics, 7: 231-238. 1978.
28) Zhang X, Rutherford MS, Shook LB. Characterization of SLA-DRB and SLA-DQB alleles distinguished by PCR-RFLP. Proceeding of XXVth International Conference on Animal Genetics: 85. 1996.

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/03/10 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 1) Burnsberg U, Edfors-Lilja I, Andersson L, Gustafsson K. Structure and organization of pig MHC class II DRBgenes: evidence for genetic exchange between loci. Immunogenetics, 44: 1-8. 1996.
3) Conley AJ, Jung YC, Schwartz NK, Warner CM, Rothschild MF, Ford SP. Influence of SLA haplotype on ovulation rate and litter size in miniature pigs. Journal of Reproduction and Fertility, 82: 595-601. 1988.
4) Ford SP, Schwartz NK, Rothschild MF, Conley AJ, Warner CM. Influence of SLA haplotype on preimplantation embryonic cell number in miniature pigs. Journal of Reproduction and Fertility, 84: 99-104. 1988.
5) Gustafsson K, LeGuern C, Hirsch F, Germana S, Pratt K, Sachs DH. Class II genes of miniature swine. IV. Charac-terization and expres-sion of two allelic class II DQB cDNA clones. Journal of Immunology, 145: 1946-1951. 1990.
6) Gustafsson K, Germana S, Hirsch F, Pratt K, LeGuern C, Sachs DH. Structure of miniature swine class II DRBgenes: conservation of hypervariable amino acid residues between distantly related mammalian species. Proceedingsof the National Academy of Sciences, USA, 87: 9798-9802. 1990.
7) Hirsch F, Sachs DH, Gustafsson K, Pratt K, Germana S, LeGuern C. Class II genes of miniature swine. III. haracerization of an expressed pig class II gene homologous to HLA-DQA. Immunogenetics, 31: 52-56. 1990.
8) Hirsch F, Germana S, Gustafsson K, Pratt K, Sachs DH, LeGuern C. Structure and expression of Class II α genes in miniature swine. Journal of Immunology, 149: 841-846. 1992.
9) Hughes AL, Nei M. Nucleotide substitution at the major histocompatibility complex II loci: Evidence for over-dominant selection. Proceedings of the National Academy of Sciences, USA, 86: 958-962. 1989.
11) Klein J. Natural History of the Major Histocompatibility Complex. 423-607. John Wiley & Sons, Inc. New York.1986.
13) Lacey C, Wilkie BN, Kennedy BW, Mallard BA. Genetic and other effects on bacterial phagocytosis and killing bycultured peripheral blood monocytes of SLA-defined miniature pigs. Animal Genetics, 20: 371-382. 1989.
14) Lumsden JS, Kennedy BW, Mallard BA, Wilkie BN. The influence of the swine major histocompatibility genes onantibody and cell mediated immune responses to immunization with an aromatic-dependent mutant of Salmonellatyphimurium. Canadian Journal of Veterinary Research, 57: 14-18. 1993.
15) Lunney JK, Pescovitz MD, Sachs DH. The swine major histocompati-bility complex: its structure and function.In: Swine in Biomedical Research vol. 3. (Tumbleson ME ed.) 1821-1835. Plenum Press. New York. 1986.
16) Madden KB, Moeller RF, Douglass LW, Goldman T, Lunney JK. Trichinella spiralis: Genetic basis and kineticsof the anti-encrysted muscle larval response in miniature swine. Experimental Parasitology, 77: 23-25. 1993.
17) Mallard BA, Wilkie BN, Kennedy BW. Genetic and other effects on antibody and cell mediated immune response inswine leucocyte antigen (SLA )-defined miniature pigs. Animal Genetics, 20: 167-178. 1989.
18) Mallard BA, Wilkie BN, Kennedy BW. The influence of the swine major histocompatibility genes (SLA) on varia-tion in serum immunoglobulin (Ig) concentration. Veterinary Immunology and Immunopathology, 21: 139-151. 1989.
19) Nielsen VH, Thomsen B. Pvu II RFLPs at SLA class II loci: DQA and DRA. Animal Genetics, 28: 151. 1997.
20) Peigiong L. Xiang pig: A superior raw material for special pork products (A review). Proceedings of the International Symposium on Chinese Pig Breeds: 525-529. 1992.
21) Prather RS, Gibson J, Parry TW, Hoffman KE. Reproductive measurements in Sinclair and NIH miniature pigs: aretrospective analysis. Theriogenology, 47: 433-440. 1997.
22) Renard C, Vaiman M. Possible relationships between SLA and porcine reproduction. Reproduction, Nutrition and Development, 29: 569-576. 1989.
23) Rothschild MF, Chen HL, Christian LL, Lie WR, Venier L, Cooper M, Briggs C, Warner CM. Breed and winelymphocyte antigen haplotype differences in agglutination titers following vaccination with B. Bronchiseptica. Journal of Animal Science, 59: 643-649. 1984.
24) Shia YC, Bradshaw M, Rutherford MS, Lewin HA, Schook LB. Polymerase chain reaction based genotyping for char-acterization of SLA-DQB and SLA-DRB alleles in domestic pigs. Animal Genetics, 26: 91-100. 1995.
25) Tanaka K, Oishi T, Kurosawa Y, Suzuki S. Genetic relationship among several pig populations in East Asiaanalysed by blood groups and serum protein polymorphism. Animal Blood Groups and Biochemical Genetics, 14: 191-200. 1983.
26) Vage DI, Olsaker I, Lingaas F, Lie O. Isolation and sequence determination of porcine class II DRB allelesamplified by PCR. Animal Genetics, 25: 73-75. 1994.
27) Vaiman M, Metzger J, Renard C, Vila J. Immune response gene (s) controlling the humoral anti-lysozyme response(Ir-Lys) linked to the major histocompatibility complex SLA in the pig. Immunogenetics, 7: 231-238. 1978.
28) Zhang X, Rutherford MS, Shook LB. Characterization of SLA-DRB and SLA-DQB alleles distinguished by PCR-RFLP. Proceeding of XXVth International Conference on Animal Genetics: 85. 1996.

Right : 1) Burnsberg U, Edfors-Lilja I, Andersson L, Gustafsson K. Structure and organization of pig MHC class II DRBgenes: evidence for genetic exchange between loci. Immunogenetics, 44: 1-8. 1996.
3) Conley AJ, Jung YC, Schwartz NK, Warner CM, Rothschild MF, Ford SP. Influence of SLA haplotype on ovulation rate and litter size in miniature pigs. Journal of Reproduction and Fertility, 82: 595-601. 1988.
4) Ford SP, Schwartz NK, Rothschild MF, Conley AJ, Warner CM. Influence of SLA haplotype on preimplantation embryonic cell number in miniature pigs. Journal of Reproduction and Fertility, 84: 99-104. 1988.
5) Gustafsson K, LeGuern C, Hirsch F, Germana S, Pratt K, Sachs DH. Class II genes of miniature swine. IV. Charac-terization and expres-sion of two allelic class II DQB cDNA clones. Journal of Immunology, 145: 1946-1951. 1990.
6) Gustafsson K, Germana S, Hirsch F, Pratt K, LeGuern C, Sachs DH. Structure of miniature swine class II DRBgenes: conservation of hypervariable amino acid residues between distantly related mammalian species. Proceedingsof the National Academy of Sciences, USA, 87: 9798-9802. 1990.
7) Hirsch F, Sachs DH, Gustafsson K, Pratt K, Germana S, LeGuern C. Class II genes of miniature swine. III. haracerization of an expressed pig class II gene homologous to HLA-DQA. Immunogenetics, 31: 52-56. 1990.
8) Hirsch F, Germana S, Gustafsson K, Pratt K, Sachs DH, LeGuern C. Structure and expression of Class II α genes in miniature swine. Journal of Immunology, 149: 841-846. 1992.
9) Hughes AL, Nei M. Nucleotide substitution at the major histocompatibility complex II loci: Evidence for over-dominant selection. Proceedings of the National Academy of Sciences, USA, 86: 958-962. 1989.
11) Klein J. Natural History of the Major Histocompatibility Complex. 423-607. John Wiley & Sons, Inc. New York.1986.
13) Lacey C, Wilkie BN, Kennedy BW, Mallard BA. Genetic and other effects on bacterial phagocytosis and killing bycultured peripheral blood monocytes of SLA-defined miniature pigs. Animal Genetics, 20: 371-382. 1989.
14) Lumsden JS, Kennedy BW, Mallard BA, Wilkie BN. The influence of the swine major histocompatibility genes onantibody and cell mediated immune responses to immunization with an aromatic-dependent mutant of Salmonellatyphimurium. Canadian Journal of Veterinary Research, 57: 14-18. 1993.
15) Lunney JK, Pescovitz MD, Sachs DH. The swine major histocompati-bility complex: its structure and function.In: Swine in Biomedical Research vol. 3. (Tumbleson ME ed.) 1821-1835. Plenum Press. New York. 1986.
16) Madden KB, Moeller RF, Douglass LW, Goldman T, Lunney JK. Trichinella spiralis: Genetic basis and kineticsof the anti-encrysted muscle larval response in miniature swine. Experimental Parasitology, 77: 23-25. 1993.
17) Mallard BA, Wilkie BN, Kennedy BW. Genetic and other effects on antibody and cell mediated immune response inswine leucocyte antigen (SLA )-defined miniature pigs. Animal Genetics, 20: 167-178. 1989.
18) Mallard BA, Wilkie BN, Kennedy BW. The influence of the swine major histocompatibility genes (SLA) on varia-tion in serum immunoglobulin (Ig) concentration. Veterinary Immunology and Immunopathology, 21: 139-151. 1989.
19) Nielsen VH, Thomsen B. Pvu II RFLPs at SLA class II loci: DQA and DRA. Animal Genetics, 28: 151. 1997.
20) Peigiong L. Xiang pig: A superior raw material for special pork products (A review). Proceedings of the International Symposium on Chinese Pig Breeds: 525-529. 1992.
21) Prather RS, Gibson J, Parry TW, Hoffman KE. Reproductive measurements in Sinclair and NIH miniature pigs: aretrospective analysis. Theriogenology, 47: 433-440. 1997.
22) Renard C, Vaiman M. Possible relationships between SLA and porcine reproduction. Reproduction, Nutrition and Development, 29: 569-576. 1989.
23) Rothschild MF, Chen HL, Christian LL, Lie WR, Venier L, Cooper M, Briggs C, Warner CM. Breed and winelymphocyte antigen haplotype differences in agglutination titers following vaccination with B. Bronchiseptica. Journal of Animal Science, 59: 643-649. 1984.
24) Shia YC, Bradshaw M, Rutherford MS, Lewin HA, Schook LB. Polymerase chain reaction based genotyping for char-acterization of SLA-DQB and SLA-DRB alleles in domestic pigs. Animal Genetics, 26: 91-100. 1995.
25) Tanaka K, Oishi T, Kurosawa Y, Suzuki S. Genetic relationship among several pig populations in East Asiaanalysed by blood groups and serum protein polymorphism. Animal Blood Groups and Biochemical Genetics, 14: 191-200. 1983.
26) Vage DI, Olsaker I, Lingaas F, Lie O. Isolation and sequence determination of porcine class II DRB allelesamplified by PCR. Animal Genetics, 25: 73-75. 1994.
27) Vaiman M, Metzger J, Renard C, Vila J. Immune response gene (s) controlling the humoral anti-lysozyme response(Ir-Lys) linked to the major histocompatibility complex SLA in the pig. Immunogenetics, 7: 231-238. 1978.
28) Zhang X, Rutherford MS, Shook LB. Characterization of SLA-DRB and SLA-DQB alleles distinguished by PCR-RFLP. Proceeding of XXVth International Conference on Animal Genetics: 85. 1996.

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/03/10 訂正理由: - 訂正箇所: 発行日訂正内容: 訂正後 : 20000225

訂正日: 2008/03/10 訂正理由: - 訂正箇所: 所属機関情報訂正内容: Wrong :
1) Chugoku National Experiment Station
2) Tokyo Metropolitan Livestock Station
3) Kagoshima prefectural Institute of Animal Science
4) STAFF Institute
5) University of Philippines Los Banos

Right :
2) Tokyo Metropolitan Livestock Station
3) Kagoshima prefectural Institute of Animal Science
4) STAFF Institute
5) University of Philippines Los Banos
6) Chugoku National Experiment Station

訂正日: 2008/03/10 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

我々が先に報告したRT-PCR-RFLP法によるSLAクラスII遺伝子のDNAタイピング法を用いて,フィリピン在来豚,中国産香猪由来小型豚,北京黒豚,バークシャー,梅山豚,デュロック,ランドレース,ユカタンマイクロ豚8品種55頭のSLAクラスII遺伝子3種類(SLA-DRB1,-DQBおよび-DQA)のDNAタイピングを行い,本方法が各種ブタ品種のSLAクラスII遺伝子のDNAタイピング法として有効であることを確認した.さらに,Shiaらの方法および本方法によるSLA-DRB1および-DQB両遺伝子のRFLPバンドパターンを統一した.本方法により,現在まで報告されている55種類のSLA-DRB1遺伝子は22種類のグループに,24種類のSLA-DQB遺伝子は23種類のグループに各々分類できた.SLA-DQA遺伝子は4種類のグループに分類できた.また,本方法により新たに3種類のSLADRB1遺伝子グループおよび9種類のSLA-DQB遺伝子グループを分類した.さらに,各SLAクラスII遺伝子の品種による特徴を明らかにした.

責任著者(Corresponding author)

訂正情報

J-STAGEへの登録はこちら（無料）