2004 年 52 巻 10 号 p. 1251-1254
In the present study, a reversed phase high performance liquid chromatographic (RP-HPLC) method was established for simultaneous determination of chlorogenic acid, caffeic acid, ferulic acid, protocatechuic acid and protocatechuic aldehyde in a Chinese herbal preparation (Fufang-Pugongying-Mixture). The separation was performed on a Hypersil ODS-2 column by isocratic elution with methanol and 0.2 M acetate buffer (pH 3.6) (15 : 85, v/v) as the mobile phase at the flow-rate of 1.0 ml/min with operating temperature of 30 °C, and detection wavelength of 300 nm. A good linear regression relationship between peak-areas and concentrations was obtained over the range of 2—200 μg/ml for the five marker compounds mentioned above. The spike recoveries were within 96.72—104.07%. The variation coefficient (CV) values of the precision were in the range of 0.89—4.50%. Moreover the developed method has reference value for quantitative analysis of Taraxacum, Lonicera and Angelica.
