A Novel Fluorescent Probe for Zinc Ion Based on Boron Dipyrromethene (BODIPY) Chromophore

Hitomi Koutaka; Jun-ichi Kosuge; Noboru Fukasaku; Tomoya Hirano; Kazuya Kikuchi; Yasuteru Urano; Hirotatsu Kojima; Tetsuo Nagano

doi:10.1248/cpb.52.700

Regular Articles

A Novel Fluorescent Probe for Zinc Ion Based on Boron Dipyrromethene (BODIPY) Chromophore

Hitomi Koutaka, Jun-ichi Kosuge, Noboru Fukasaku, Tomoya Hirano, Kazuya Kikuchi, Yasuteru Urano, Hirotatsu Kojima, Tetsuo Nagano

著者情報

キーワード: zinc, fluorescence, boron dipyrromethene (BODIPY), photoinduced electron transfer, N,N-bis(2-pyridylmethyl)ethylenediamine

ジャーナルフリー

2004 年 52 巻 6 号 p. 700-703

DOI https://doi.org/10.1248/cpb.52.700

詳細

抄録

ZnAB has the combined structure of N,N-bis(2-pyridylmethyl)ethylenediamine as a specific chelater for Zn²⁺ and 1,3,5,7-tetramethyl-8-phenyl-boron dipyrromethene as a fluorophore. Complexation of ZnAB with Zn²⁺ produces a remarkable enhancement of fluorescence intensity. ZnAB has the advantages of less sensitivity to solvent polarity and pH than fluorescein-based Zn²⁺ probes. Furthermore, it is not influenced by other cations, such as Na⁺, K⁺, Ca²⁺, and Mg²⁺, which exist at high concentrations under physiological conditions, even at 2.5 mM. The results show that ZnAB is a Zn²⁺ probe suitable for biological applications.

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