Synthesis of New Galβ1→4Fuc Segments Useful for Biological Investigations

Kazusa Nishiyama; Atsushi Yamada; Tomoharu Takeuchi; Yoichiro Arata; Ken-ichi Kasai; Tetsuta Oshitari; Hideaki Natsugari; Hideyo Takahashi

doi:10.1248/cpb.59.1307

Notes

Synthesis of New Galβ1→4Fuc Segments Useful for Biological Investigations

Kazusa Nishiyama, Atsushi Yamada, Tomoharu Takeuchi, Yoichiro Arata, Ken-ichi Kasai, Tetsuta Oshitari, Hideaki Natsugari, Hideyo Takahashi

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Keywords: galectin, annexin, chemical probe, affinity chromatography

JOURNAL FREE ACCESS

2011 Volume 59 Issue 10 Pages 1307-1310

DOI https://doi.org/10.1248/cpb.59.1307

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Abstract

Useful segments (1, 2) for chemical probes embedded in a Galβ1→4Fuc unit were designed and prepared for characterizing sugar-binding proteins in Caenorhabditis elegans. Segment 1 with an amino group terminus was used as a recognition unit in affinity chromatography. It was revealed that some proteins (annexins and galectins) in C. elegans have an affinity for Galβ1→4Fuc.

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