Abstract
The biliary excretion of DAB metabolites was first examined by paper chromatography, colorimetry, and by other methods. Presence of a selectivity in rat liver was recognized such as the main excretion of DAB metabolites retaining the azo bond into the bile and seven kinds of DAB metabolites (AB, 4'-OS-DAB, 4'-OS-MAB, 4'-OS-AB, 4'-OG-MAB, 4'-OG-MAB, 4'-OG-AB) were identified. Second, influence of DAB feeding on the amount of these seven kinds of DAB metabolites was examined by the method of newly devised separatory determination using DEAE ion-exchanger and other methods. The most appropriate explanation for the results obtained was the promotion of hydroxylation in the first and middle stages of DAB feeding, although there were yet several problems.
