Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Fluorometric Determination of Cyanide by the Reaction with Pyridoxal
高梨 茂田村 善蔵
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1970 年 18 巻 8 号 p. 1633-1635

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Cyanide produces a fluorescent compound from pyridoxal (PAL) by a catalytic action. The velocity of the reaction could be measured by measuring the fluorescence intensity. For a concentration of cyanide below 10-5M, the apparent initial velocity of the reaction was maintained for 60 min at a concentration of 6×10-4M of PAL, and the initial velocity was proportional to the concentration of cyanide. A simple and sensitive fluorometric method for the determination of cyanide was devised based on the above findings. Cyanide was treated with PAL at pH 7.5 and the fluorescence intensity was measured at pH 10.
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© The Pharmaceutical Society of Japan
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