A Fluorophotometric Method for the Determination of Pyridoxal and Its Phosphate by Using Glycine and Zinc (II) Ion
1976 年 24 巻 5 号 p. 1094-1097
詳細
1976 年 24 巻 5 号 p. 1094-1097
Pyridoxal (PL) or pyridoxal phosphate (PLP) reacts with glycine in the presence of zinc (II) ion in pyridine-methanol and gives a highly fluorescence with excitation maximum at 390nm and emission maximum at 475nm. The fluorogen of this reaction is N-pyridoxylidene zinc (II) chelate. Nanomoles of PL or PLP can be estimated spectrofluorophotometrically by using this reaction which is also very specific. Other amino acids, sugars and related substances caused no influence on the determination of PL or PLP. Application of the method to biological samples, such as blood and tissue, is suggested.
