Biosynthesis of Puerarin in Pueraria Root

Abstract

^<14>C-Labelled isoliquiritigenin was efficiently incorporated into puerarin of Pueraria lobata but ¹⁴C-labelled daidzein was almost not into it. These results support that the C-glycosylation occurs at the stage of chalcone or flavanone. Further competitive feeding experiments with T-or ¹⁴C-labelled isoliquiritigenin and liquiritigenin suggest that Cglycosylchalcone would be a possible intermediate for the biosynthesis of puerarin.