Abstract
New derivatization of primary and secondary amines for electrochemical detection in liquid chromatography has been developed. The derivatization reagents were prepared from vanillic acid and homovanillic acid by condensation with N-hydroxysuccinimide. Of the two reagents the activated ester of homovanillic acid reacted more readily with amines, providing the amides which were highly responsible for an electrochemical detector in reversed phase chromatography. The detection and quantitation limits determined with ethyl phenylalaninate were 200 pg and 1 ng, respectively.
